编码大鼠神经细胞黏附分子L1的cDNA的分子克隆。细胞质区域中的两种L1同工型是通过差异剪接产生的。

Molecular cloning of cDNA encoding the rat neural cell adhesion molecule L1. Two L1 isoforms in the cytoplasmic region are produced by differential splicing.

作者信息

Miura M, Kobayashi M, Asou H, Uyemura K

机构信息

Department of Physiology, Keio University School of Medicine, Tokyo, Japan.

出版信息

FEBS Lett. 1991 Sep 2;289(1):91-5. doi: 10.1016/0014-5793(91)80915-p.

DOI:10.1016/0014-5793(91)80915-p

PMID:1894011

Abstract

We have isolated and sequenced a full-length cDNA encoding the rat neural cell adhesion molecule L1. The deduced amino acid sequence as a whole shows high homology to mouse L1 sequence. In addition to this complete form of L1, we found an isoform, L1cs, which lacks four amino acid residues (RSLE) in the cytoplasmic domain and probably is derived from the same single L1 gene by tissue-specific alternative splicing. While L1 mRNA was predominantly expressed in the brain, L1cs mRNA was found exclusively in peripheral nervous tissue. Differential splicing in the highly conserved cytoplasmic domain may play an important role in modulating the function of L1 in different cells.

摘要

我们已经分离并测序了编码大鼠神经细胞黏附分子L1的全长cDNA。推导得到的氨基酸序列整体与小鼠L1序列具有高度同源性。除了这种完整形式的L1，我们还发现了一种异构体L1cs，它在细胞质结构域中缺少四个氨基酸残基（RSLE），可能是由同一单一L1基因通过组织特异性可变剪接产生的。虽然L1 mRNA主要在脑中表达，但L1cs mRNA仅在外周神经组织中发现。高度保守的细胞质结构域中的可变剪接可能在调节L1在不同细胞中的功能方面发挥重要作用。