Jin Hong, Ma Kai-dong, Hu Rong, Chen Yan, Yang Fengying, Yao Jun, Li Xiao-tian, Yang Peng-yuan
Department of Chemistry, Fudan University, Shanghai 200433, China.
Anal Chim Acta. 2008 Nov 23;629(1-2):158-64. doi: 10.1016/j.aca.2008.09.015. Epub 2008 Sep 12.
Preeclampsia (PE) is a complex and serious condition of pregnancy. Trophoblasts in human placenta can be separated and collected by laser capture micro-dissection (LCM). Protein in trophoblasts have been extracted and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), finally 962 unique proteins are identified by liquid chromatography coupled to mass spectrometry (LC-MS/MS). Comparison of differential expressed proteins in normal and those in PE are investigated. Two-dimensional electrophoresis (2DE) and MS were used to identify differential expressed proteins. 13 differential expressed proteins include signal transduction protein, molecular chaperone, cell skeleton proteins are identified, in which 3 proteins are down-regulated and 10 proteins are up-regulated. They might be correlated with the cause of PE.
子痫前期（PE）是一种复杂且严重的妊娠病症。人胎盘的滋养层细胞可通过激光捕获显微切割（LCM）进行分离和收集。滋养层细胞中的蛋白质已通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）进行提取和分离，最终通过液相色谱-质谱联用（LC-MS/MS）鉴定出962种独特的蛋白质。对正常妊娠和子痫前期妊娠中差异表达蛋白质进行了比较研究。采用二维电泳（2DE）和质谱法鉴定差异表达蛋白质。鉴定出13种差异表达蛋白质，包括信号转导蛋白、分子伴侣、细胞骨架蛋白，其中3种蛋白质表达下调，10种蛋白质表达上调。它们可能与子痫前期的病因相关。
