Wang Gaoxue, Zhou Zhuang, Cheng Chao, Yao Jiayun, Yang Zhengwei
College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi 712100, China.
Vet Parasitol. 2008 Nov 25;158(1-2):144-51. doi: 10.1016/j.vetpar.2008.07.034. Epub 2008 Aug 5.
The efficacy of two active compounds, isolated from the fruit of Fructus cnidii, was determined against Dactylogyrus intermedius in goldfish (Carassius auratus). The chemical structure of the two compounds was identified by spectral analysis. Designated Compounds I and II, they were isolated by successive silica gel column chromatography and used, in combination, in an in vivo anthelmintic efficacy assay. At dose rates of 1.6 and 9.5 mg l(-1), respectively, the two compounds were 100% effective against D. intermedius compared to mebendazole (positive control), used at a concentration of 1.5 mg l(-1), which is 100% efficacious. Compounds I and II were shown to be safe for goldfish at dose rates of up to 6.4 and 12.5 mg l(-1), respectively. The median effective dose (ED(50)) of Compounds I and II for D. intermedius-infested goldfish after 48 h exposure was 0.807 and 8.050 mg l(-1), respectively. If the median lethal dose (LD(50)) values for goldfish of the two compounds are 6.749 and 12.759 mg l(-1), respectively, the ED(50) values for D. intermedius yielded a therapeutic index of 8.14 and 1.52 for Compounds I and II, respectively, at 48 h. Compounds I and II were identified as osthol and isopimpinellin by physico-chemical detection.
测定了从蛇床子果实中分离出的两种活性化合物对金鱼（Carassius auratus）体内中型指环虫（Dactylogyrus intermedius）的疗效。通过光谱分析确定了这两种化合物的化学结构。这两种化合物分别命名为化合物I和化合物II，通过连续硅胶柱色谱法分离得到，并联合用于体内驱虫疗效试验。与浓度为1.5 mg l(-1)、驱虫效果达100%的甲苯咪唑（阳性对照）相比，这两种化合物在剂量率分别为1.6和9.5 mg l(-1)时，对中型指环虫的驱虫效果均达100%。结果表明，化合物I和化合物II在剂量率分别高达6.4和12.5 mg l(-1)时对金鱼是安全的。在暴露48小时后，化合物I和化合物II对感染中型指环虫的金鱼的半数有效剂量（ED(50)）分别为0.807和8.050 mg l(-1)。如果这两种化合物对金鱼的半数致死剂量（LD(50)）值分别为6.749和12.759 mg l(-1)，那么在48小时时，化合物I和化合物II对中型指环虫的ED(50)值分别产生了8.14和1.52的治疗指数。通过理化检测，化合物I和化合物II被鉴定为蛇床子素和异茴芹素。
