Phytopathology. 2002 Mar;92(3):294-9. doi: 10.1094/PHYTO.2002.92.3.294.
ABSTRACT Components of resistance to the faba bean rust (Uromyces viciae-fabae) were studied at the histological level in seedlings and adult plants of nine faba bean (Vicia faba) lines differing in their level of resistance. Resistance of these lines was previously shown to be characterized macroscopically by an increased latent period, a decreased colony size, and a relatively decreased infection frequency. In some lines, the resistance also was associated with macroscopically visible necrosis. Histological investigations revealed few differences in spore germination and appressorium formation. Significant levels of aborted stomatal penetration by the rust fungus were found on all resistant lines. However, differences among lines were more evident once the stomata were penetrated by the infection structures. Resistance was mainly due to a restriction of haustorium formation with varying levels of early abortion of the colonies, a reduction in the number of haustoria per colony, and smaller colony size. In addition, necrosis of the host cells associated with infection hyphae was detectable in some lines from the beginning of colony development. This microscopically visible necrosis became stronger from 4 days after inoculation, resulting in a reduced growth of the colony. Differences in resistance levels were more marked in adult plants than in seedlings.
摘要 在组织学水平上，研究了 9 个不同水平抗蚕豆锈病（Uromyces viciae-fabae）的蚕豆（Vicia faba）品系的幼苗和成年植株的抗性。这些品系的抗性以前被宏观特征所描述，表现为潜伏期延长、菌落大小减小和相对感染频率降低。在一些品系中，抗性也与宏观可见的坏死有关。组织学研究表明，孢子萌发和附着胞形成差异很小。在所有抗性品系上，都发现锈菌对气孔的侵入有较高水平的中止。然而，一旦感染结构穿透气孔，品系之间的差异就更加明显。抗性主要归因于吸器形成的限制，其特征是菌落早期流产的程度不同，每个菌落的吸器数量减少，以及菌落大小减小。此外，在一些品系中，从菌落发育开始就可以检测到与感染菌丝相关的宿主细胞坏死。这种在显微镜下可见的坏死从接种后 4 天开始增强，导致菌落生长减少。与幼苗相比，成年植株的抗性水平差异更为明显。
