Phytopathology. 2006 Jan;96(1):96-104. doi: 10.1094/PHYTO-96-0096.
ABSTRACT Sequence-tagged microsatellite profiling was used to develop 110 microsatellites for Puccinia graminis f. sp. tritici (causal agent of wheat stem rust). Low microsatellite polymorphism was exhibited among 10 pathogenically diverse P. graminis f. sp. tritici isolates collected from Australian cereal growing regions over a period of at least 70 years, with two polymorphic loci detected, each revealing two alleles. Limited cross-species amplification was observed for the wheat rust pathogens, P. triticina (leaf rust) and P. striiformis f. sp. tritici (stripe rust). However, very high transferability was revealed with P. graminis f. sp. avenae (causal agent of oat stem rust) isolates. A genetic diversity study of 47 P. graminis f. sp. avenae isolates collected from an Australia-wide survey in 1999, and a historical group of 16 isolates collected from Australian cereal growing regions from 1971 to 1996, revealed six polymorphic microsatellite loci with a total of 15 alleles. Genetic analysis revealed the presence of several clonal lineages and subpopulations in the pathogen population, and wide dispersal of identical races and genotypes throughout Australian cereal-growing regions. These findings demonstrated the dynamic population structure of this pathogen in Australia and concur with the patterns of diversity observed in pathogenicity studies.
摘要 利用序列标记微卫星分析技术，为小麦叶锈菌（ Puccinia graminis f. sp. tritici ）开发了 110 个微卫星。从澳大利亚的谷物种植区采集的至少 70 年时间内具有不同致病性的 10 个小麦叶锈菌（ Puccinia graminis f. sp. tritici ）分离株显示出低微卫星多态性，仅检测到两个多态性位点，每个位点揭示了两个等位基因。对小麦锈病病原体，小麦叶锈菌（ P. triticina ）和条锈病（ P. striiformis f. sp. tritici ），观察到有限的跨种扩增。然而，与燕麦冠锈菌（ P. graminis f. sp. avenae ）分离株的交叉种转移能力非常高。对 1999 年澳大利亚全国范围内调查收集的 47 个燕麦冠锈菌（ P. graminis f. sp. avenae ）分离株和 1971 年至 1996 年从澳大利亚谷物种植区收集的 16 个历史群体进行的遗传多样性研究，揭示了 6 个具有 15 个等位基因的多态性微卫星位点。遗传分析表明病原体群体中存在几个无性系和亚种群，以及相同的菌系和基因型在澳大利亚整个谷物种植区广泛传播。这些发现证明了该病原体在澳大利亚的动态种群结构，并与致病性研究中观察到的多样性模式一致。
