Phytopathology. 2002 Jun;92(6):603-9. doi: 10.1094/PHYTO.2002.92.6.603.
ABSTRACT Arrhenotokous and thelytokous populations of Thrips tabaci from tobacco or leek plants were evaluated for their ability to transmit Tomato spotted wilt virus (TSWV) and for their host preference. Transmission efficiencies were comparatively studied using leaf disks of Petunia hybrida, Datura stramonium, and Nicotiana tabacum cv. Basmas. Adults of arrhenotokous populations collected on infected tobacco plants in the field were efficient transmitters (up to 48.5% transmission) and remained so when maintained on tobacco for several generations. Arrhenotokous T. tabacipopulations from leek plants were poor transmitters (up to 3.1% transmission), whereas no transmission was obtained with thelytokous populations from leek. All populations could infest leek, however none of the arrhenotokous and thelytokous populations from leek plants was able to infest tobacco. TSWV could be acquired by both first and second larval instars of a T. tabacipopulation from tobacco. However, the transmission by adults decreased with the age at which the virus was acquired by larvae. The highest efficiencies (61% of males and 51% of females transmitted) were obtained when newborn (0- to 24-h old) larvae acquired the virus. The majority of thrips started to transmit after becoming adult and rates were positively correlated with the temperature at which the thrips were kept. The median latent period values found for adults decreased with increasing temperature. The median acquisition access period (AAP50) of the population was 41 min, whereas the AAP(50) was 65 min for males and 35 min for females. The median inoculation access period of males was 246 and 365 min on tobacco and petunia, respectively, and 96 and 345 min for females. The results show that T. tabaci forms a complex in terms of host preference, reproductive strategy, and ability to transmit TSWV. The transmission parameters show that the thrips of arrhenotokous populations infesting tobacco are highly efficient vectors.
摘要 对来自烟草或韭菜植株的单性生殖和孤雌生殖型烟粉虱种群进行了评估，以确定其传播番茄斑萎病毒（TSWV）的能力和对宿主的偏好。使用杂种矮牵牛、曼陀罗和烟草 cv. Basmas 的叶片圆盘比较研究了传病效率。在田间从受感染的烟草植株上收集的单性生殖型烟粉虱种群的成虫是有效的传播者（最高 48.5%的传播率），并且在连续几代维持在烟草上时仍然如此。来自韭菜植株的单性生殖型烟粉虱种群是低效的传播者（最高 3.1%的传播率），而来自韭菜的孤雌生殖型烟粉虱种群则没有传播。所有种群都可以侵害韭菜，但来自韭菜的单性生殖和孤雌生殖型烟粉虱种群都不能侵害烟草。TSWV 可以被来自烟草的烟粉虱种群的第一和第二龄幼虫获得。然而，成虫的传播率随幼虫获得病毒时的年龄而降低。当新生（0-24 小时龄）幼虫获得病毒时，效率最高（雄性 61%，雌性 51%传播）。大多数粉虱在成虫后开始传播，并且传播率与粉虱所处的温度呈正相关。发现成虫的中位潜伏时间值随温度升高而降低。该种群的中位获得接触期（AAP50）为 41 分钟，而雄性为 65 分钟，雌性为 35 分钟。雄性在烟草和矮牵牛上的中位接种接触期分别为 246 和 365 分钟，而雌性分别为 96 和 345 分钟。结果表明，烟粉虱在宿主偏好、生殖策略和传播 TSWV 的能力方面形成了一个复杂的群体。这些传播参数表明，侵染烟草的单性生殖型烟粉虱种群是高效的传播媒介。
