Phytopathology. 2000 May;90(5):546-52. doi: 10.1094/PHYTO.2000.90.5.546.
Phylogenetic and distance analyses place Chino del tomate virus (CdTV) in the New World clade of begomoviruses and indicate that CdTV and Tomato leaf crumple virus (TLCrV) are closely related strains of the same virus. One cloned CdTV A component (pCdTV-H6), when inoculated to tomato with the B component (pCdTV-B52), produced mild symptoms and low DNA titers. Another cloned CdTV A component (pCdTV-H8), when coinoculated to tomato with the B component, produced moderate leaf curling and veinal chlorosis similar to that of TLCrV. Coinoculation of both CdTV A components and the B component to tomato produced wild-type chino del tomate (CdT) disease symptoms consisting of severe leaf curling, veinal and interveinal chlorosis, and stunting. The two CdTV A components were nearly identical, except at nucleotide positions 1,722 and 2,324. The polymorphism at nucleotide 1,722 resulted in a change at Rep amino acid 261. The second polymorphism at nucleotide 2,324 resulted in changes at Rep amino acid 60 and AC4 amino acid 10. Two chimeric A components constructed by reciprocal exchange of a fragment bearing the polymorphic site at nucleotide 1,722 were evaluated for symptom phenotype. One chimeric A component (pCdTV-H86) produced wild-type CdT symptoms when coinoculated to tomato with the B component. The reciprocal chimeric A component (pCdTV-H68), when coin-oculated to tomato with the B component, also produced severe leaf curling, veinal chlorosis, and stunting. However, pCdTV-H68 induced less obvious interveinal chlorosis than wild-type or pCdTV-H86. Examination of A component genotypes recovered from tomato coinoculated with pCdTV-H6 and pCdTV-H8 indicated that recombination occurred to produce a genotype identical to pCdTV-H86. These results indicate that subtle genotypic variation has significant effects on symptom expression and may explain phenotypic differences observed among isolates and cloned DNAs of CdTV and TLCrV.
系统发生和距离分析将番茄斑萎病毒（CdTV）置于新世界伴生病毒组中，并表明 CdTV 和番茄皱叶病毒（TLCrV）是同一病毒的密切相关株系。克隆的 CdTV A 组分（pCdTV-H6）与 B 组分（pCdTV-B52）共接种番茄时，产生轻度症状和低 DNA 滴度。另一个克隆的 CdTV A 组分（pCdTV-H8）与 B 组分共接种番茄时，产生中度叶片卷曲和叶脉失绿，与 TLCrV 相似。将两个 CdTV A 组分和 B 组分共接种番茄会产生典型的番茄斑萎病（CdT）症状，包括严重叶片卷曲、叶脉和脉间失绿以及矮化。这两个 CdTV A 组分几乎相同，只有在核苷酸位置 1722 和 2324 处存在差异。核苷酸 1722 处的多态性导致 Rep 氨基酸 261 发生变化。核苷酸 2324 处的第二个多态性导致 Rep 氨基酸 60 和 AC4 氨基酸 10 发生变化。通过交换带有核苷酸 1722 处多态性位点的片段构建的两个嵌合 A 组分进行了症状表型评估。当与 B 组分共接种番茄时，一个嵌合 A 组分（pCdTV-H86）产生典型的 CdT 症状。当与 B 组分共接种番茄时，其对应的嵌合 A 组分（pCdTV-H68）也产生严重的叶片卷曲、叶脉失绿和矮化。然而，与典型或 pCdTV-H86 相比，pCdTV-H68 诱导的间脉失绿不那么明显。对用 pCdTV-H6 和 pCdTV-H8 共接种番茄回收的 A 组分基因型的检查表明，重组发生产生了与 pCdTV-H86 相同的基因型。这些结果表明，细微的基因型变异对症状表达有显著影响，并可能解释 CdTV 和 TLCrV 分离株和克隆 DNA 之间观察到的表型差异。
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