Hernández-Zepeda Cecilia, Idris Ali M, Carnevali Germán, Brown Judith K, Moreno-Valenzuela Oscar A
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán A. C., Merida, Yucatan, Mexico.
Virus Genes. 2007 Oct;35(2):369-77. doi: 10.1007/s11262-007-0080-5. Epub 2007 Feb 15.
Sida acuta and Corchorus siliquosus plants showing yellow mosaic and yellow vein symptoms, respectively, were collected in the Yucatan Peninsula, Mexico. Total DNA was isolated from both plant species and used for the amplification, cloning, and sequencing of the Begomovirus genome. Nucleotide comparison of the complete DNA-A component isolated from S. acuta and C. siliquosus confirmed the presence of two distinct begomoviruses species. Based on phenotypic symptoms observed in infected field plants, the names Sida yellow mosaic Yucatan virus (SiYMYuV) and Corchorus yellow vein Yucatan virus (CoYVYuV) were proposed. The SiYMYuV DNA-A shared the highest nucleotide identity (86%) with the Okra yellow mosaic Mexico virus (OkYMMV). The complete DNA-B component shared the highest nucleotide identity (80%) with CoYVYuV. The CoYVYuV DNA-A shared the highest nucleotide identity (84%) with SiYMYuV. The 166-nt common region (CR) sequence for the DNA-A and DNA-B components of SiYMYuV shared a high nucleotide identity of 99%, and the 151 nt of CoYVYuV CR shared 95% of nucleotide identity. The organization and the iterated sequence of the putative AC1 binding site (located within the common region) of both isolates, were similar to that of the begomoviruses of the Western Hemisphere. Phylogenetic analyses placed the DNA-A and DNA-B of SiYMYuV and CoYVYuV in the clade containing the Abutilon mosaic virus (AbMV).
分别在墨西哥尤卡坦半岛采集到表现出黄色花叶症状的刺蒴麻植株和表现出黄色叶脉症状的长角黄麻植株。从这两种植物中分离出总DNA，并用于双生病毒基因组的扩增、克隆和测序。对从刺蒴麻和长角黄麻中分离出的完整DNA-A组分进行核苷酸比较，证实存在两种不同的双生病毒物种。根据在感染田间植株上观察到的表型症状，提出了刺蒴麻黄色花叶尤卡坦病毒（SiYMYuV）和长角黄麻黄色叶脉尤卡坦病毒（CoYVYuV）这两个名称。SiYMYuV的DNA-A与秋葵黄色花叶墨西哥病毒（OkYMMV）的核苷酸同一性最高（86%）。完整的DNA-B组分与CoYVYuV的核苷酸同一性最高（80%）。CoYVYuV的DNA-A与SiYMYuV的核苷酸同一性最高（84%）。SiYMYuV的DNA-A和DNA-B组分的166个核苷酸的共同区域（CR）序列具有99%的高核苷酸同一性，CoYVYuV的CR的151个核苷酸具有95%的核苷酸同一性。两种分离物推定的AC1结合位点（位于共同区域内）的组织和重复序列与西半球双生病毒的相似。系统发育分析将SiYMYuV和CoYVYuV的DNA-A和DNA-B置于包含苘麻花叶病毒（AbMV）的进化枝中。
