Phytopathology. 1999 Nov;89(11):1084-7. doi: 10.1094/PHYTO.1999.89.11.1084.
ABSTRACT Seed health tests are usually performed on a sample of a seed lot; therefore, it is crucial that the test sample be as homogeneous as possible and representative of the lot. Seed sampling procedures appropriate for seed health testing have been developed by seed testing organizations such as the International Seed Testing Association and the Association of Official Seed Analysts. Seed lot size is generally not a constraint when the distribution of contaminated/infected seed in the lot is relatively homogeneous; if the distribution is heterogeneous, increased sampling intensity is required. Sample size is determined by the damage threshold (intolerable infection level) for the pathogen and the probability of detection desired, commonly 95 or 99%. The probability of detection at a given damage threshold is greater as sample size increases, and this probability and the appropriate sample size can be determined by statistical methods. Most seed health tests utilize qualitative data based on the presence or absence of the pathogen in the test sample, with the lot being rejected if the pathogen is detected in the sample and accepted if the sample is negative.
摘要 种子健康测试通常在种子批的一个样本上进行；因此，测试样本尽可能均匀且具有代表性是至关重要的。种子测试组织（如国际种子测试协会和官方种子分析师协会）已经制定了适用于种子健康测试的种子抽样程序。如果种子批中污染/感染种子的分布相对均匀，则种子批大小通常不是限制因素；如果分布不均匀，则需要增加抽样强度。样本大小由病原体的损伤阈值（不可容忍的感染水平）和所需的检测概率决定，通常为 95%或 99%。在给定的损伤阈值下，随着样本量的增加，检测概率越大，并且可以通过统计方法确定该概率和适当的样本量。大多数种子健康测试都利用基于病原体在测试样本中存在或不存在的定性数据，如果在样本中检测到病原体，则该批种子将被拒绝，如果样本为阴性，则该批种子将被接受。
