Phytopathology. 1999 May;89(5):407-13. doi: 10.1094/PHYTO.1999.89.5.407.
ABSTRACT Fusarium oxysporum f. sp. canariensis causes Fusarium wilt disease on the Canary Island date palm (Phoenix canariensis). To facilitate disease management, a polymerase chain reaction diagnostic method has been developed to rapidly detect the pathogen. A partial genomic library of F. oxysporum f. sp. canariensis isolate 95-913 was used to identify a DNA sequence diagnostic for a lineage containing all tested isolates of F. oxysporum f. sp. canariensis. Two oligonucleotide primers were designed and used to amplify a 567-bp fragment with F. oxysporum f. sp. canariensis DNAs. DNA from 61 outgroup isolates did not amplify using these primers. Once the primers were shown to amplify a 0.567-kb fragment from DNA of all the F. oxysporum f. sp. canariensis isolates tested, a rapid DNA extraction procedure was developed that led to the correct identification of 98% of the tested F. oxysporum f. sp. canariensis isolates.
尖孢镰刀菌古巴专化型引起加那利岛枣椰树（Phoenix canariensis）枯萎病。为了便于疾病管理，已经开发了聚合酶链反应诊断方法来快速检测病原体。使用尖孢镰刀菌古巴专化型分离株 95-913 的部分基因组文库来鉴定包含所有测试的尖孢镰刀菌古巴专化型分离株的谱系的 DNA 序列诊断。设计了两个寡核苷酸引物，用于扩增具有尖孢镰刀菌古巴专化型 DNA 的 567-bp 片段。使用这些引物从 61 个外群分离物的 DNA 中未扩增出 DNA。一旦证明引物可从所有测试的尖孢镰刀菌古巴专化型分离物的 DNA 中扩增出 0.567-kb 片段，就开发了一种快速 DNA 提取程序，该程序正确鉴定了 98%的测试的尖孢镰刀菌古巴专化型分离物。
