Phytopathology. 1999 Apr;89(4):273-80. doi: 10.1094/PHYTO.1999.89.4.273.
ABSTRACT Bean calico mosaic virus (BCMoV), a whitefly-transmitted geminivirus from Sonora, Mexico, was purified, and the genome components were cloned and sequenced. Purified viral fractions and cloned genome components were infectious by biolistic inoculation to bean, completing Koch's postulates for both. The B biotype of the whitefly Bemisia tabaci efficiently transmitted both native virus and progeny virus derived from cloned DNA inoculum. Host ranges of native virus and of progeny virus derived from cloned DNA were identical based upon whitefly and biolistic mediated transmission, respectively. BCMoV has a relatively wide experimental host range among begomoviruses known to infect bean, encompassing genera and species within the Fabaceae, Malvaceae, and Solanaceae. BCMoV has a bipartite genome, as do other New World begomoviruses. BCMoV DNA-A shared highest nucleotide sequence identities with squash leaf curl virus-E strain (SLCV-E) and cabbage leaf curl virus (CaLCV) at 80.1 and 80.7%, respectively. BCMoV DNA-B shared highest nucleotide sequence identity with SLCV-E at 70.7%. The common region (CR) sequences of BCMoV and SLCV-E are 73 to 76% identical; however, modular cis-acting elements within the CR involved in replication origin function and recognition are 100% conserved. Phy-logenetic analysis indicated that BCMoV DNA-A shares a most recent common ancestor with the DNA-A of two viruses that also occur in the Sonoran Desert, SLCV-E and Texas pepper virus (TPV-TAM), and CaLCV from Florida. In contrast, a phylogenetic analysis indicated that BCMoV DNA-B shares a most recent common ancestor with SLCV-E; whereas DNA-B of CaLCV clustered in a separate clade with pepper hausteco virus. Collectively, biological and molecular characteristics indicate that BCMoV is a distinct begomovirus species with the northernmost distribution of any begomovirus isolated from bean in the Americas. Furthermore, the phylogenetic relationships of begomovirus cognate components are not necessarily identical, suggesting that DNA-A and DNA-B of some begomoviruses may have different evolutionary histories.
摘要 来自墨西哥索诺拉的豆科蚜传双生病毒（BCMoV）已被纯化，并对其基因组成分进行了克隆和测序。通过生物弹射击接种，纯化的病毒部分和克隆的基因组成分均具有感染性，从而完成了两者的科赫假设。烟粉虱的 B 型有效地传播了天然病毒和源自克隆 DNA 接种物的后代病毒。基于烟粉虱和生物弹接种介导的传播，天然病毒和源自克隆 DNA 的后代病毒的宿主范围是相同的。BCMoV 在已知感染豆类的茄科病毒中具有相对较宽的实验宿主范围，包括豆科、锦葵科和茄科的属和种。BCMoV 具有二分体基因组，与其他新世界的伴生病毒相同。BCMoV DNA-A 与 squash leaf curl virus-E 株（SLCV-E）和 cabbage leaf curl virus（CaLCV）的核苷酸序列同源性最高，分别为 80.1%和 80.7%。BCMoV DNA-B 与 SLCV-E 的核苷酸序列同源性最高，为 70.7%。BCMoV 和 SLCV-E 的共同区域（CR）序列 73%至 76%相同；然而，CR 内涉及复制起点功能和识别的模块顺式作用元件 100%保守。系统发育分析表明，BCMoV DNA-A 与也发生在索诺拉沙漠的 SLCV-E 和 Texas pepper virus（TPV-TAM）以及来自佛罗里达州的 CaLCV 的两种病毒的 DNA-A 具有最近的共同祖先。相比之下，系统发育分析表明，BCMoV DNA-B 与 SLCV-E 具有最近的共同祖先；而 CaLCV 的 DNA-B 与 pepper hausteco virus 聚类在一个单独的分支中。总之，生物学和分子特征表明，BCMoV 是一种独特的伴生病毒，是从美洲豆类中分离出的分布最北的伴生病毒。此外，伴生病毒同源成分的系统发育关系不一定相同，这表明一些伴生病毒的 DNA-A 和 DNA-B 可能具有不同的进化历史。
