Phytopathology. 1998 Nov;88(11):1224-30. doi: 10.1094/PHYTO.1998.88.11.1224.
ABSTRACT Closterovirus-like particles associated with mealybug wilt of pineapple were acquired and transmitted by the pink pineapple mealybug, Dysmicoccus brevipes, and the gray pineapple mealybug, D. neobrevipes. Mealybugs acquired pineapple mealybug wilt-associated virus (PMWaV) from infected pineapple plants or detached leaves. The virus was detected in plants by tissue blot immunoassay and confirmed by immunosorbent electron microscopy. Plants exposed to mealybugs reared on PMWaV-free pineapple tissue remained uninfected. The presence of ants was correlated with an increased rate of virus spread when caged with D. brevipes. All stages of D. neobrevipes acquired PMWaV, although vector efficiency decreased significantly in older adult females. The probability of a single third-instar immature transmitting the virus was 0.04. Both species of mealybug acquired and transmitted PMWaV from infected pineapple material that had been clonally propagated for decades, and both species acquired PMWaV from sources previously infected with the virus by the other mealybug species.
摘要 与菠萝绵粉蚧萎蔫病相关的杆状病毒样颗粒由粉蚧、短须粉蚧和灰粉蚧、新菠萝绵粉蚧获得和传播。粉蚧从感染的菠萝植株或离体叶片中获得菠萝绵粉蚧萎蔫相关病毒（PMWaV）。通过组织印迹免疫测定法在植物中检测到该病毒，并通过免疫吸附电子显微镜进行确认。暴露于饲养在无 PMWaV 菠萝组织上的粉蚧的植物仍然未感染。当与短须粉蚧一起饲养时，有蚂蚁存在与病毒传播率的增加相关。新菠萝绵粉蚧的所有阶段都获得了 PMWaV，尽管老年雌成虫的媒介效率显著下降。单个三龄若虫传播病毒的概率为 0.04。两种粉蚧都从已克隆繁殖数十年的感染菠萝材料中获得和传播 PMWaV，并且两种粉蚧都从以前被另一种粉蚧感染的来源中获得 PMWaV。
