Phytopathology. 1998 Jun;88(6):514-9. doi: 10.1094/PHYTO.1998.88.6.514.
ABSTRACT Four natural variants of satellite tobacco mosaic virus (STMV) were compared with each other and with the type strain. Differences were detected in double-stranded RNA, single-stranded RNA, and virion electrophoretic mobility patterns, while the size and antigenicity of the coat protein were similar for all. RNase protection assays detected differences in the genomes of each of the four new variants, which differed not only from each other, but also from that of type STMV. Infectious RNA transcripts were made from complementary DNA clones of one variant (STMV 10) with a genome apparently smaller than that of type STMV. A 71-base deletion in the region that contains the 6.8-kDa protein in type STMV was detected by sequence analysis of the STMV 10 clones, a result that is confirmed by the lack of a 6.8-kDa in vitro translation product for STMV 10. Only minor sequence differences exist elsewhere in the genome compared with that of type STMV. Type STMV and STMV 10 each successfully cross-protected against the other when tobacco plants were inoculated 10 days apart.
摘要 对 4 种天然卫星烟草花叶病毒（STMV）变体进行了相互比较，并与标准株进行了比较。在双链 RNA、单链 RNA 和病毒粒子电泳迁移率模式方面存在差异，而所有变体的外壳蛋白大小和抗原性相似。RNase 保护测定检测到 4 种新变体的基因组存在差异，它们不仅彼此不同，而且与标准 STMV 也不同。从一个变体（STMV10）的 cDNA 克隆中合成了具有明显小于标准 STMV 基因组的传染性 RNA 转录物。通过对 STMV10 克隆的序列分析，检测到标准 STMV 中包含 6.8kDa 蛋白的区域存在 71 个碱基缺失，这一结果通过 STMV10 的体外翻译产物中缺乏 6.8kDa 蛋白得到证实。与标准 STMV 相比，基因组的其他区域仅存在较小的序列差异。当烟草植物在相隔 10 天接种时，标准 STMV 和 STMV10 都能成功地对彼此产生交叉保护作用。
