Phytopathology. 1998 Jun;88(6):520-4. doi: 10.1094/PHYTO.1998.88.6.520.
ABSTRACT Three hundred thirty-eight isolates of cucumber mosaic cucumovirus (CMV), sampled from natural populations in six areas of Spain between 1989 and 1996, were screened for the presence of satellite RNA (satRNA). The frequency of CMV isolates with satRNA approached 1.00 in Valencia (east Spain) between 1990 and 1994 where a tomato necrosis epidemic induced by CMV+satRNA had started in 1986 and was smaller north and west of this area in 1992 and 1993. After 1994, satRNA almost disappeared from all CMV populations. Genetic typing of satRNA variantswas done by ribonuclease protection assay, and from these data, genetic distances were estimated for any pair of satRNA variants. CMV-satRNA populations were highly diverse, containing 0.07865 nucleotide substitutions per site on average. Data also showed that the whole compared set of 100 satRNA variants form a single population that is not structured according to place, year, host plant, or strain of helper virus (HV). This is in sharp contrast with the metapopulation structure of the Spanish CMV population. Thus, the genetic structure and dynamics of populations of CMV and its satRNA are not coupled. This shows that CMV-satRNA spreads epidemically, as a hyperparasite, in the population of its HV. This conclusion is relevant to the use of CMV-satRNA as a biocontrol agent of CMV.
摘要 1989 年至 1996 年期间，从西班牙六个地区的自然种群中采集了 338 株黄瓜花叶黄瓜花叶病毒（CMV）分离物，对其是否存在卫星 RNA（satRNA）进行了筛选。在 1990 年至 1994 年期间，瓦伦西亚（西班牙东部）CMV 分离物携带 satRNA 的频率接近 1.00，1986 年该地区爆发了由 CMV+satRNA 引起的番茄坏死病，1992 年和 1993 年该地区北部和西部的情况较小。1994 年后，satRNA 几乎从所有 CMV 种群中消失。通过核糖核酸酶保护测定法对 satRNA 变体进行遗传分型，并根据这些数据，估算了任何一对 satRNA 变体之间的遗传距离。CMV-satRNA 群体高度多样化，平均每个位点含有 0.07865 个核苷酸取代。数据还表明，比较的整个 100 个 satRNA 变体集形成一个单一的群体，根据地点、年份、宿主植物或辅助病毒（HV）的菌株，没有结构。这与西班牙 CMV 种群的复合种群结构形成鲜明对比。因此，CMV 和其 satRNA 群体的遗传结构和动态没有耦合。这表明 CMV-satRNA 作为 HV 的超寄生生物，在其 HV 种群中呈流行传播。这一结论与将 CMV-satRNA 用作 CMV 的生物防治剂有关。
