Phytopathology. 1998 Jul;88(7):658-65. doi: 10.1094/PHYTO.1998.88.7.658.
ABSTRACT The virulence of Meloidogyne hapla, M. chitwoodi, and M. fallax was studied on genotypes of Solanum spp. in a greenhouse. Juveniles of 11 M. hapla race A isolates, 3 M. hapla race B isolates, and 5 mono-female lines of a M. hapla race A isolate were inoculated on S. chacoense, S. hougasii, and S. sparsipilum. Juveniles of eight M. chitwoodi isolates, five M. fallax isolates, and six mono-female lines of a M. chitwoodi isolate were inoculated on S. bulbocastanum, S. chacoense, S. hougasii, S. stoloniferum, and S. tuberosum. Virulence was expressed as nematode reproduction 8 weeks after inoculation. Nematode reproduction was estimated by the number of egg masses and, in one experiment, by the number of hatched second-stage juveniles per inoculated juvenile. Considerable variation in virulence and resistance was observed among M. hapla isolates and plant genotypes, respectively. The M. hapla isolate-plant species interaction was highly significant. The response to M. chitwoodi ranged from susceptible (S. tuberosum and S. chacoense) to highly resistant (S. bulbocastanum and S. hougasii). S. tuberosum was susceptible to M. fallax, whereas all four wild species were resistant. In contrast to M. hapla, no significant isolate-plant genotype interaction was obtained for M. chitwoodi or M. fallax, indicating no or little intraspecific variation in virulence. M. chitwoodi juveniles in species mixtures with M. fallax isolates appeared to be able to break the resistance of S. bulbocastanum and S. hougasii. Significant differences among mono-female lines of M. hapla and M. chitwoodi were observed, indicating heterogeneity of pathogenicity within meiotic parthenogenic Meloidogyne populations.
摘要 在温室条件下，研究了茎线虫（Meloidogyne hapla）、M. chitwoodi 和 M. fallax 在茄属基因型上的毒力。将 11 个 M. hapla 种群 A 的分离物、3 个 M. hapla 种群 B 的分离物和 1 个 M. hapla 种群 A 的 5 个单雌系的幼虫接种到 S. chacoense、S. hougasii 和 S. sparsipilum 上。将 8 个 M. chitwoodi 分离物、5 个 M. fallax 分离物和 1 个 M. chitwoodi 分离物的 6 个单雌系的幼虫接种到 S. bulbocastanum、S. chacoense、S. hougasii、S. stoloniferum 和 S. tuberosum 上。8 周后，通过接种幼虫的卵块数量和孵化的第二龄幼虫数量来评估线虫的繁殖情况。线虫的繁殖力用接种幼虫的卵块数量表示，在一个实验中用孵化的第二龄幼虫数量表示。在 M. hapla 分离物和植物基因型之间分别观察到毒力和抗性的差异。M. hapla 分离物-植物种间的相互作用非常显著。M. chitwoodi 的反应范围从易感（S. tuberosum 和 S. chacoense）到高度抗性（S. bulbocastanum 和 S. hougasii）。S. tuberosum 易受 M. fallax 感染，而所有四个野生种均具有抗性。与 M. hapla 不同，对于 M. chitwoodi 和 M. fallax，未获得或仅获得轻微的分离物-植物基因型互作，表明毒力没有或仅有很少的种内变异。在 M. chitwoodi 分离物与 M. fallax 分离物的物种混合物中，M. chitwoodi 幼虫似乎能够打破 S. bulbocastanum 和 S. hougasii 的抗性。观察到 M. hapla 和 M. chitwoodi 的单雌系之间存在显著差异，表明减数分裂孤雌生殖的茎线虫种群在致病性上存在异质性。
