Phytopathology. 1997 Jul;87(7):772-9. doi: 10.1094/PHYTO.1997.87.7.772.
ABSTRACT The effect of 2-deoxy-D-glucose on major postharvest pathogens was investigated at the ultrastructural and cytochemical level. Hyphae of Botrytis cinerea, Penicillium expansum,, and Rhizopus stolonifer grown in the absence of 2-deoxy-D-glucose were normal and showed no apparent cytological alterations. In the presence of 2-deoxy-D-glucose, however, these fungi exhibited severe cellular injuries ranging from cell wall disruption to cytoplasm disintegration. Although 2-deoxy-D-glucose caused cytoplasmic degeneration in the three fungi tested, cell wall alterations were exhibited only by B. cinerea and R. stolonifer. In the latter, the retraction of degenerated cytoplasm was often accompanied by the deposition of amorphous material in paramural spaces. Cytochemical study of fungal cell wall components showed that 2-deoxy-D-glucose caused a marked increase of chitin- and beta-1,3-glucan-labeling in R. stolonifer and B. cinerea, indicating an interference of 2-deoxy-D-glucose with fungal wall biosynthesis. The observed cellular alterations indicate that 2-deoxy-D-glucose may also have affected other metabolic processes.
摘要 在超微结构和细胞化学水平上研究了 2-脱氧-D-葡萄糖对主要采后病原菌的影响。在不存在 2-脱氧-D-葡萄糖的情况下生长的灰葡萄孢菌、扩展青霉和腐生毛霉的菌丝正常，没有明显的细胞学改变。然而，在 2-脱氧-D-葡萄糖存在的情况下，这些真菌表现出严重的细胞损伤，从细胞壁破裂到细胞质解体。尽管 2-脱氧-D-葡萄糖导致了三种测试真菌的细胞质退化，但只有灰葡萄孢菌和腐生毛霉表现出细胞壁改变。在后一种真菌中，退化细胞质的收缩常伴有无定形物质在壁旁空间的沉积。真菌细胞壁成分的细胞化学研究表明，2-脱氧-D-葡萄糖导致腐生毛霉和灰葡萄孢菌中壳聚糖和β-1,3-葡聚糖标记明显增加，表明 2-脱氧-D-葡萄糖干扰了真菌细胞壁的生物合成。观察到的细胞改变表明，2-脱氧-D-葡萄糖可能还影响了其他代谢过程。
