使用LightCycler平台通过实时一步法逆转录聚合酶链反应检测腮腺炎病毒RNA。
Detection of mumps virus RNA by real-time one-step reverse transcriptase PCR using the LightCycler platform.
作者信息
Leblanc Jason J, Pettipas Janice, Davidson Ross J, Tipples Graham A, Hiebert Joanne, Hatchette Todd F
机构信息
Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada.
出版信息
J Clin Microbiol. 2008 Dec;46(12):4049-51. doi: 10.1128/JCM.01446-08. Epub 2008 Oct 22.
Abstract
A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.
摘要
一种针对临床样本中的腮腺炎病毒F基因和人类核糖核酸酶P的实时逆转录聚合酶链反应（RT-PCR）检测方法被适配用于LightCycler平台。LightCycler RT-PCR与传统巢式RT-PCR一样灵敏，且成本显著降低，劳动强度也大大减轻，使其成为疫情期间腮腺炎诊断的理想方法。
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