Tenuta L M A, Cerezetti R V, Del Bel Cury A A, Tabchoury C P M, Cury J A
Piracicaba Dental School, UNICAMP, Av. Limeira 901, 13414-903, Piracicaba, SP Piracicaba, SP, Brazil.
J Dent Res. 2008 Nov;87(11):1032-6. doi: 10.1177/154405910808701105.
The anticaries effect of professional fluoride (F) application has been attributed to calcium-fluoride-like deposits (CaF(2)) formed on enamel, but this has not been clearly demonstrated. We hypothesized that CaF(2) formed on plaque-free enamel by F application would reduce enamel demineralization due to the increase of F availability in fluid of subsequently formed plaque. We created distinct levels of CaF(2) on enamel to evaluate a dose-response effect. Enamel blocks were mounted in contact with a S. mutans test plaque and used in situ by 10 volunteers. F released to the fluid phase of this substrate ("plaque fluid") was measured before a cariogenic challenge. "Plaque fluid" F concentration was highly correlated to the enamel CaF(2) concentration (r = 0.96, p < 0.001) and to consequent enamel demineralization (r = -0.75, p < 0.001). The results suggest that F released to plaque fluid from CaF(2) formed on enamel may play a significant role in the anticaries effect of professionally applied F agents.
专业应用氟化物（F）的防龋作用归因于在牙釉质上形成的类氟化钙沉积物（CaF₂），但这一点尚未得到明确证实。我们推测，通过应用氟化物在无菌斑牙釉质上形成的CaF₂会减少牙釉质脱矿，这是因为随后形成的菌斑液中氟的可用性增加。我们在牙釉质上形成不同水平的CaF₂，以评估剂量反应效应。将牙釉质块与变形链球菌测试菌斑接触放置，并由10名志愿者进行原位使用。在致龋攻击前测量释放到该底物液相（“菌斑液”）中的氟。“菌斑液”中的氟浓度与牙釉质CaF₂浓度高度相关（r = 0.96，p < 0.001），并与随后的牙釉质脱矿相关（r = -0.75，p < 0.001）。结果表明，从牙釉质上形成的CaF₂释放到菌斑液中的氟可能在专业应用氟制剂的防龋作用中发挥重要作用。
