Walsh F S, Cronin M, Koblar S, Doherty P, Winer J, Leon A, Hughes R A
Department of Experimental Pathology, U.M.D.S., Guy's Hospital, London, U.K.
J Neuroimmunol. 1991 Oct;34(1):43-51. doi: 10.1016/0165-5728(91)90097-q.
In a retrospective study, we have analysed sera from a well-characterised Guillain-Barré syndrome (GBS) patient group for antibodies that react with gangliosides. Of 95 GBS patients and 85 control patients analysed, we found that 14 (15%) of GBS patients but only one control patient had antibodies that react with the gangliosides GM1 and/or GD1b but not GM2, GD1a and GT1b using a sensitive enzyme-linked immunosorbent assay (ELISA). This pattern of reactivity suggests binding to the carbohydrate structure Gal(beta 1-3)GalNAc which is shared between some glycolipids and glycoproteins. Similar antibodies have been found previously in a subpopulation of patients with lower motor neuron disease. In the present study, the predominant immunoglobulin class of these anti-glycoconjugate antibodies was IgG rather than IgM. A correlation was found between the presence of these antibodies and prognosis in terms of disability at 3 and 12 months after presentation. Patients with anti-glycoconjugate antibodies also had a higher incidence of previous Campylobacter infections than the rest of the patient group, although the significance of this remains to be determined.
在一项回顾性研究中，我们分析了一组特征明确的吉兰-巴雷综合征（GBS）患者血清中与神经节苷脂发生反应的抗体。在分析的95例GBS患者和85例对照患者中，我们发现，使用灵敏的酶联免疫吸附测定（ELISA）法，14例（15%）GBS患者有与神经节苷脂GM1和/或GD1b发生反应但不与GM2、GD1a和GT1b发生反应的抗体，而对照患者中只有1例有此类抗体。这种反应模式表明抗体与一些糖脂和糖蛋白共有的碳水化合物结构Gal（β1-3）GalNAc结合。此前在一组下运动神经元疾病患者中也发现了类似抗体。在本研究中，这些抗糖缀合物抗体的主要免疫球蛋白类别为IgG而非IgM。发现这些抗体的存在与发病后3个月和12个月时的残疾预后之间存在相关性。抗糖缀合物抗体的患者先前感染空肠弯曲菌的发生率也高于其余患者组，不过其意义尚待确定。
