Tal H, Kozlovsky A, Pitaru S
Department of Periodontology, Maurice and Gabriela Goldschieger School of Dental Medicine, Tel Aviv University, Israel.
J Clin Periodontol. 1991 Aug;18(7):543-7. doi: 10.1111/j.1600-051x.1991.tb00087.x.
The potential of periodontal ligament-derived tissues to regenerate periodontal attachment after cryosurgical trauma to the PDL in dogs was evaluated. The buccal alveolar plate of each canine tooth was exposed by a semi-lunar excision. A 3 mm thick cryoprobe, cooled to -81 degrees C, was placed on the bone 5 mm apical to the crest for 10 s. This induced cellular devitalization in the bone directly in contact with the probe and the PDL under it. The freezing-thawing cycle was repeated 3 times. Control sites were sham-operated at room temperature. Histologic sections from the center of the lesions were obtained from 1 h, 48 h and 30 d specimens. 1-h control and experimental histologic sections were similar. At 48 h post-surgery, the cellular component of the frozen PDL could not be identified and inflammatory response was minimal. The collagenous framework, however, appeared to form a continuum between the alveolar bone and cementum. Lacunae in the bone at the frozen segment were empty. The injured PDL was surrounded by normal PDL. Control specimens appeared normal. At 30 d, the PDL space in the frozen segments was populated by PDL-like tissue which did not differ significantly from the PDL coronal or apical to it. Collagen fibers appeared to be attached to the cementum on one side and to the alveolar bone on the other. Bone resorption or ankylosis was not observed in the experimental sites. It is suggested that the extracellular matrix in the devitalized area was preserved, supporting regeneration of the cryolesion.
评估了犬牙周膜来源组织在牙周膜冷冻手术创伤后再生牙周附着的潜力。通过半月形切除术暴露每颗犬齿的颊侧牙槽骨板。将冷却至-81℃的3mm厚冷冻探头置于牙槽嵴顶下方5mm的骨上10秒。这导致与探头直接接触的骨以及其下方的牙周膜细胞失活。冻融循环重复3次。对照部位在室温下进行假手术。从1小时、48小时和30天的标本中获取病变中心的组织学切片。1小时的对照和实验组织学切片相似。术后48小时，冷冻牙周膜的细胞成分无法识别，炎症反应轻微。然而，胶原纤维框架似乎在牙槽骨和牙骨质之间形成了连续体。冷冻段骨中的骨陷窝是空的。受损的牙周膜被正常的牙周膜包围。对照标本看起来正常。在30天时，冷冻段的牙周膜间隙中充满了类似牙周膜的组织，与该组织冠方或根方的牙周膜没有显著差异。胶原纤维似乎一侧附着于牙骨质，另一侧附着于牙槽骨。在实验部位未观察到骨吸收或骨粘连。提示失活区域的细胞外基质得以保留，支持冷冻损伤的再生。
