Li Hai-Xia, Mao Xu-Hu, Shi Yun, Ma Ying, Wu Ya-Nan, Zhang Wei-Jun, Luo Ping, Yu Shu, Zhou Wei-Ying, Guo Ying, Wu Chao, Guo Gang, Zou Quan-Ming
Department of Clinical Microbiology and Clinical Immunology, The Third Military Medical University, Chongqing 400038, People's Republic of China.
Vaccine. 2008 Dec 9;26(52):6945-9. doi: 10.1016/j.vaccine.2008.09.089. Epub 2008 Oct 21.
Urease plays a crucial role in the survival and pathogenesis of Helicobacter pylori (H. pylori), and antibody neutralizing the urease activity may be implicated for the protection against H. pylori infection. Previously, a neutralizing monoclonal antibody (MAb) 6E6 against UreB of H. pylori was developed. In this work, we try to identify the B-cell epitope recognized by neutralizing MAb 6E6. Following screening a series of truncated proteins of UreB, an epitope was primarily localized in the aa 200-230 of UreB. Subsequently, we screened the overlapping synthetic peptides covering the aa 200-230 and identified a novel B-cell epitope (U(211-225), IEAGAIGFKIHEDWG) that was recognized by specific MAb 6E6. The newly identified epitope may help understanding of the protective immunity against H. pylori and be implicated for vaccine development.
脲酶在幽门螺杆菌（H. pylori）的生存和致病过程中起着关键作用，中和脲酶活性的抗体可能对预防H. pylori感染具有保护作用。此前，已开发出一种针对H. pylori尿素酶B亚基（UreB）的中和单克隆抗体（MAb）6E6。在本研究中，我们试图鉴定中和性MAb 6E6识别的B细胞表位。在筛选了一系列UreB截短蛋白后，一个表位初步定位在UreB的第200 - 230位氨基酸处。随后，我们筛选了覆盖第200 - 230位氨基酸的重叠合成肽，并鉴定出一个新的B细胞表位（U(211 - 225)，IEAGAIGFKIHEDWG），该表位可被特异性MAb 6E6识别。新鉴定的表位可能有助于理解针对H. pylori的保护性免疫，并对疫苗开发具有启示意义。
