生物传感器板可在体内检测线粒体生理调节因子和突变。
Biosensor plates detect mitochondrial physiological regulators and mutations in vivo.
作者信息
Rolo Anabela P, Palmeira Carlos M, Cortopassi Gino A
机构信息
Center for Neurosciences and Cell Biology, Department of Zoology, University of Coimbra, 3004-517 Coimbra, Portugal.
出版信息
Anal Biochem. 2009 Feb 1;385(1):176-8. doi: 10.1016/j.ab.2008.10.002. Epub 2008 Oct 7.
Abstract
The measurement of mitochondrial activity in living cells is usually not straightforward, even though it is quite important in physiological and pathophysiological processes. We describe a high-throughput method for measurement of mitochondrial oxygen consumption in living cells, based on the Becton-Dickinson Biosensor plates.
摘要
尽管线粒体活性的测量在生理和病理生理过程中非常重要，但在活细胞中进行该测量通常并非易事。我们描述了一种基于贝克顿-迪金森生物传感器板的高通量方法，用于测量活细胞中的线粒体耗氧量。
相似文献
1
Biosensor plates detect mitochondrial physiological regulators and mutations in vivo.生物传感器板可在体内检测线粒体生理调节因子和突变。
Anal Biochem. 2009 Feb 1;385(1):176-8. doi: 10.1016/j.ab.2008.10.002. Epub 2008 Oct 7.
2
Measuring Respiration in Isolated Murine Brain Mitochondria: Implications for Mechanistic Stroke Studies.测量分离的鼠脑线粒体中的呼吸：对机制性卒中研究的影响。
Neuromolecular Med. 2019 Dec;21(4):493-504. doi: 10.1007/s12017-019-08552-8. Epub 2019 Jun 6.
3
Disruption of the human COQ5-containing protein complex is associated with diminished coenzyme Q10 levels under two different conditions of mitochondrial energy deficiency.在两种不同的线粒体能量缺乏条件下，含人类COQ5的蛋白质复合物的破坏与辅酶Q10水平降低有关。
Biochim Biophys Acta. 2016 Sep;1860(9):1864-76. doi: 10.1016/j.bbagen.2016.05.005. Epub 2016 May 4.
4
Rhodamine-based sensor for real-time imaging of mitochondrial ATP in living fibroblasts.基于若丹明的传感器，用于实时成像活成纤维细胞中线粒体 ATP。
Biochim Biophys Acta Bioenerg. 2017 Dec;1858(12):999-1006. doi: 10.1016/j.bbabio.2017.09.004. Epub 2017 Sep 22.
5
Uncoupling protein-1 is not leaky.解偶联蛋白-1并非渗漏性的。
Biochim Biophys Acta. 2010 Jun-Jul;1797(6-7):773-84. doi: 10.1016/j.bbabio.2010.04.007. Epub 2010 Apr 14.
6
Differential role of Presenilin-1 and -2 on mitochondrial membrane potential and oxygen consumption in mouse embryonic fibroblasts.早老素-1和-2对小鼠胚胎成纤维细胞线粒体膜电位和氧消耗的不同作用。
J Neurosci Res. 2006 Sep;84(4):891-902. doi: 10.1002/jnr.20990.
7
Contribution of diffusion to the oxygen dependence of energy metabolism in human neuroblastoma cells.扩散对人神经母细胞瘤细胞能量代谢氧依赖性的贡献。
Adv Exp Med Biol. 1989;248:829-33. doi: 10.1007/978-1-4684-5643-1_94.
8
Assay of mitochondrial functions by resazurin in vitro.体外使用刃天青检测线粒体功能
Acta Pharmacol Sin. 2004 Mar;25(3):385-9.
9
Structural investigations on the mitochondrial uncouplers niclosamide and FCCP.对米托坦和 FCCP 这两种线粒体解偶联剂的结构研究。
FEBS Open Bio. 2024 Jul;14(7):1057-1071. doi: 10.1002/2211-5463.13817. Epub 2024 May 15.
10
Comparative bioenergetic assessment of transformed cells using a cell energy budget platform.利用细胞能量预算平台对转化细胞进行比较生物能量评估。
Integr Biol (Camb). 2011 Nov;3(11):1135-42. doi: 10.1039/c1ib00050k. Epub 2011 Oct 17.
引用本文的文献
1
Increased Energy Expenditure, Ucp1 Expression, and Resistance to Diet-induced Obesity in Mice Lacking Nuclear Factor-Erythroid-2-related Transcription Factor-2 (Nrf2).缺乏核因子红细胞 2 相关转录因子 2（Nrf2）的小鼠能量消耗增加、Ucp1 表达增加及对饮食诱导肥胖的抵抗作用
J Biol Chem. 2016 Apr 1;291(14):7754-66. doi: 10.1074/jbc.M115.673756. Epub 2016 Feb 3.
2
Dysregulation of glutamine transporter SNAT1 in Rett syndrome microglia: a mechanism for mitochondrial dysfunction and neurotoxicity.雷特综合征小胶质细胞中谷氨酰胺转运体SNAT1的失调：线粒体功能障碍和神经毒性的一种机制
J Neurosci. 2015 Feb 11;35(6):2516-29. doi: 10.1523/JNEUROSCI.2778-14.2015.
3
High-throughput screening of FDA-approved drugs using oxygen biosensor plates reveals secondary mitofunctional effects.使用氧生物传感器板进行高通量筛选 FDA 批准的药物揭示了次要的线粒体功能效应。
Mitochondrion. 2014 Jul;17:116-25. doi: 10.1016/j.mito.2014.07.002. Epub 2014 Jul 14.
4
Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells.测量培养细胞（包括人类多能干细胞和分化细胞）的能量代谢。
Nat Protoc. 2012 May 10;7(6):1068-85. doi: 10.1038/nprot.2012.048.
5
Synthesis and characterization of mitoQ and idebenone analogues as mediators of oxygen consumption in mitochondria.线粒体耗氧量调节剂米托醌和艾地苯醌类似物的合成与表征。
Bioorg Med Chem. 2010 Sep 1;18(17):6429-41. doi: 10.1016/j.bmc.2010.06.104. Epub 2010 Aug 4.
本文引用的文献
1
Analysis of mitochondrial function using phosphorescent oxygen-sensitive probes.使用磷光氧敏感探针分析线粒体功能。
Nat Protoc. 2006;1(6):2563-72. doi: 10.1038/nprot.2006.351.
2
Rosiglitazone induces mitochondrial biogenesis in mouse brain.罗格列酮可诱导小鼠大脑中的线粒体生物合成。
J Alzheimers Dis. 2007 Mar;11(1):45-51. doi: 10.3233/jad-2007-11108.
3
Investigation of drug-induced mitochondrial toxicity using fluorescence-based oxygen-sensitive probes.使用基于荧光的氧敏感探针研究药物诱导的线粒体毒性。
Toxicol Sci. 2006 Jul;92(1):186-200. doi: 10.1093/toxsci/kfj208. Epub 2006 Apr 25.
4
Mitochondrial DNA and disease.线粒体DNA与疾病
Ann Med. 2005;37(3):222-32. doi: 10.1080/07853890510007368.
5
Increased oxygen consumption rates in response to high glucose detected by a novel oxygen biosensor system in non-human primate and human islets.通过一种新型氧生物传感器系统在非人灵长类动物和人类胰岛中检测到，对高葡萄糖的反应中氧消耗率增加。
J Endocrinol. 2005 Jun;185(3):445-55. doi: 10.1677/joe.1.06092.
6
Method for determining oxygen consumption rates of static cultures from microplate measurements of pericellular dissolved oxygen concentration.通过微孔板测量细胞周围溶解氧浓度来测定静态培养物耗氧率的方法。
Biotechnol Bioeng. 2004 Jun 30;86(7):775-87. doi: 10.1002/bit.20072.
7
Fluorescence-based cell viability screening assays using water-soluble oxygen probes.使用水溶性氧探针的基于荧光的细胞活力筛选测定法。
J Biomol Screen. 2003 Jun;8(3):264-72. doi: 10.1177/1087057103008003004.
8
Mitochondrial respiratory-chain diseases.线粒体呼吸链疾病
N Engl J Med. 2003 Jun 26;348(26):2656-68. doi: 10.1056/NEJMra022567.
9
Reproducible quantitative PCR of mitochondrial and nuclear DNA copy number using the LightCycler.使用LightCycler进行线粒体和核DNA拷贝数的可重复性定量PCR
Methods Mol Biol. 2002;197:129-37. doi: 10.1385/1-59259-284-8:129.
10
Novel fluorescent technology platform for high throughput cytotoxicity and proliferation assays.用于高通量细胞毒性和增殖测定的新型荧光技术平台。
J Biomol Screen. 2000 Jun;5(3):141-52. doi: 10.1177/108705710000500306.
Zotero 插件