Hfaiedh Najla, Allagui Mohamed Salah, Hfaiedh Mbarka, Feki Abdelfattah El, Zourgui Lazhar, Croute Françoise
Unité de recherche Biochimie macromoléculaire et Génétique, Faculté des Sciences, 2133 Gafsa, Tunisia.
Food Chem Toxicol. 2008 Dec;46(12):3759-63. doi: 10.1016/j.fct.2008.09.059. Epub 2008 Oct 4.
The purpose of this study carried out on male Wistar rats, was to evaluate the protective effects of regular ingestion of juice from the prickly pear cactus (Opuntia ficus indica) cladodes against nickel chloride toxicity. Rats were given either normal tap water or water containing 25% of cactus juice for one month. Then, rats of each group were injected daily, for 10 days, with either NiCl(2) solution (4mg (30micromol)/kg body weight) or with the same volume of saline solution (300mM NaCl). Significant increases of lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase activities and of cholesterol, triglycerides and glucose levels were observed in blood of nickel-treated rats. In the liver, nickel chloride was found to induce an oxidative stress evidenced by an increase in lipid peroxidation and changes in antioxidant enzymes activities. Superoxide-dismutase (SOD) activity was found to be increased whereas glutathione peroxidase and catalase activities were decreased. These changes did not occur in animals previously given cactus juice, demonstrating a protective effect of this vegetal extract.
本研究以雄性Wistar大鼠为对象，旨在评估定期摄入仙人掌（仙人掌属植物）茎汁对氯化镍毒性的保护作用。大鼠饮用普通自来水或含25%仙人掌汁的水，持续一个月。然后，每组大鼠每天注射10天，一组注射NiCl₂溶液（4mg（30微摩尔）/千克体重），另一组注射相同体积的盐溶液（300mM NaCl）。在镍处理大鼠的血液中，观察到乳酸脱氢酶、天冬氨酸转氨酶、丙氨酸转氨酶活性以及胆固醇、甘油三酯和葡萄糖水平显著升高。在肝脏中，发现氯化镍会诱导氧化应激，表现为脂质过氧化增加和抗氧化酶活性改变。超氧化物歧化酶（SOD）活性增加，而谷胱甘肽过氧化物酶和过氧化氢酶活性降低。这些变化在预先饮用仙人掌汁的动物中未出现，表明这种植物提取物具有保护作用。
