Bist Renu, Bhatt Devendra Kumar
Department of Bioscience and Biotechnology, Banasthali University, Banasthali (Raj), India.
J Neurol Sci. 2009 Jan 15;276(1-2):99-102. doi: 10.1016/j.jns.2008.09.008. Epub 2008 Oct 23.
The objective of the present study was to evaluate the neurotoxic effects of lindane, in mice and the protective potential of two antioxidants alpha-lipoic acid (ALA) and vitamin E, against the observed lindane induced toxicity. 7-8 weeks old healthy Swiss mice were administered acute doses of lindane (40 mg/kg b.w.) or antioxidants or both subcutaneously and analyzed 18 h later. ALA and vitamin E were used in the combination for neuroprotection in the concentration of 20 mg/kg b.w. and 50 mg/kg b.w. respectively. Lipid peroxidation, gamma-amino butyric acid (GABA) and serotonin level were used as biochemical test of toxic action for lindane induced acute toxicity. Protective effects of ALA and vitamin E were also evaluated on the same parameters. Reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) level served as an index for determining the extent of lipid peroxidation. Treatment of lindane to normal control animals resulted in a significant decrease and increase in GSH (P<0.01) and TBARS level (P<0.01) respectively in crude homogenate of whole brain. Antioxidants treatment significantly altered the level of GSH (P<0.01) and TBARS (P<0.01). GABA and serotonin level in whole brain as well as in different regions of brain were measured. Main brain regions under the investigation were olfactory lobe, cerebrum, hippocampus-hypothalamus, cerebellum and pons-medulla. Critical difference (CD) of GABA level in various groups was found significant at 1% in cerebrum and hippocampus-hypothalamus, at 5% in whole brain, cerebellum and pons-medulla (i.e. P<0.01 and P<0.05 respectively). Change in serotonin level in whole brain as well as in all studied brain regions of various groups was found significant at 1% CD (i.e. P<0.01).
本研究的目的是评估林丹对小鼠的神经毒性作用，以及两种抗氧化剂α-硫辛酸（ALA）和维生素E对所观察到的林丹诱导毒性的保护潜力。将7-8周龄的健康瑞士小鼠皮下注射急性剂量的林丹（40 mg/kg体重）或抗氧化剂或两者，18小时后进行分析。ALA和维生素E分别以20 mg/kg体重和50 mg/kg体重的浓度联合用于神经保护。脂质过氧化、γ-氨基丁酸（GABA）和血清素水平用作林丹诱导急性毒性的毒性作用生化测试。还在相同参数上评估了ALA和维生素E的保护作用。还原型谷胱甘肽（GSH）和硫代巴比妥酸反应性物质（TBARS）水平用作确定脂质过氧化程度的指标。给正常对照动物注射林丹导致全脑粗匀浆中GSH（P<0.01）和TBARS水平（P<0.01）分别显著降低和升高。抗氧化剂处理显著改变了GSH（P<0.01）和TBARS（P<0.01）的水平。测量了全脑以及脑不同区域的GABA和血清素水平。所研究的主要脑区为嗅叶、大脑、海马-下丘脑、小脑和脑桥-延髓。发现各组中GABA水平的临界差异（CD）在大脑和海马-下丘脑为1%显著，在全脑、小脑和脑桥-延髓为5%显著（即分别为P<0.01和P<0.05）。发现各组全脑以及所有研究脑区中血清素水平的变化在1% CD时显著（即P<0.01）。
