Niu Shuyan, Zhao Min, Ren Rui, Zhang Shusheng
Key Lab of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
J Inorg Biochem. 2009 Jan;103(1):43-9. doi: 10.1016/j.jinorgbio.2008.09.001. Epub 2008 Sep 18.
A Mn(II) complex, MnL (L=sodium (E)-3-((1-carboxyethylimino)methyl)-4-hydroxybenzenesulfonate), was synthesized and characterized using elemental analysis and IR spectroscopy. Cyclic voltammetry (CV) and fluorescence spectroscopy were used to investigate the interaction between MnL and salmon sperm DNA. It was revealed that MnL presented high electrochemical activity on glassy carbon electrode (GCE), and it could be intercalated into the double helices of double-stranded DNA (dsDNA). Using MnL as the hybridization indicator, a novel and sensitive electrochemical DNA biosensor based on multiwall carbon nanotubes functionalized with carboxyl groups (MWCNTs-COOH, on which DNA probes were covalently immobilized) was prepared. The target single-stranded DNA (ssDNA) could be quantified ranging from 6.7 x 10(-10)M to 8.4 x 10(-9)M with good linearity (r=0.9922). A detection limit of 1.4 x 10(-10)M (3 sigma, n=9) was achieved.
合成了一种锰（II）配合物MnL（L =（E）-3-（（1-羧乙基亚氨基）甲基）-4-羟基苯磺酸钠），并通过元素分析和红外光谱对其进行了表征。采用循环伏安法（CV）和荧光光谱法研究了MnL与鲑鱼精DNA之间的相互作用。结果表明，MnL在玻碳电极（GCE）上具有较高的电化学活性，并且可以插入双链DNA（dsDNA）的双螺旋结构中。以MnL作为杂交指示剂，制备了一种基于羧基功能化多壁碳纳米管（MWCNTs-COOH，DNA探针共价固定于其上）的新型灵敏电化学DNA生物传感器。目标单链DNA（ssDNA）的定量范围为6.7×10^(-10)M至8.4×10^(-9)M，具有良好的线性关系（r = 0.9922）。检测限达到1.4×10^(-10)M（3σ，n = 9）。
