Miklossy J, Clarke S, Van der Loos H
University Institute of Pathology, Division of Neuropathology, Lausanne, Switzerland.
J Neuropathol Exp Neurol. 1991 Sep;50(5):595-614.
This study aims at determining the reliability and the optimal post-injury survival time for the application of the Nauta technique to the analysis of the human brain. The Nauta method reveals the degeneration not only of nerve fibers, myelinated and unmyelinated, but also of their terminations. Immunohistochemical and ultrastructural observations appear to prove that the Nauta technique indeed stains axons in human autopsy material. The optimal survival time for the use of the Nauta method was found to be between nine days and five months. In cases with longer survival times--up to 20 months--the Nauta technique and a previously proposed polarizing technique (showing birefringent breakdown products of myelin) can be used as complementary methods. Applying these techniques to the human brain may help define the anatomical basis of neurological and neuropsychological symptoms important for man.
本研究旨在确定用于分析人类大脑的瑙塔技术应用的可靠性及伤后最佳存活时间。瑙塔方法不仅能揭示有髓和无髓神经纤维的变性，还能显示其终末的变性。免疫组织化学和超微结构观察似乎证明，瑙塔技术确实能对人类尸检材料中的轴突进行染色。发现使用瑙塔方法的最佳存活时间在9天至5个月之间。在存活时间较长的病例中——长达20个月——瑙塔技术和先前提出的偏振技术（显示髓磷脂的双折射分解产物）可作为补充方法使用。将这些技术应用于人类大脑可能有助于确定对人类重要的神经和神经心理症状的解剖学基础。
