Baker A, Tipping E, Thacker S A, Gondar D
School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, UK.
Chemosphere. 2008 Dec;73(11):1765-72. doi: 10.1016/j.chemosphere.2008.09.018. Epub 2008 Oct 31.
The fluorescence excitation-emission matrix properties of 25 dissolved organic matter samples from three rivers and one lake are analysed. All sites are sampled in duplicate, and the 25 samples include ten taken from the lake site, and nine from one of the rivers, to cover variations in dissolved organic matter composition due to season and river flow. Fluorescence properties are compared to the functional properties of the dissolved organic matter; the functional assays provide quantitative information on photochemical fading, buffering capacity, copper binding, benzo[a]pyrene binding, hydrophilicity and adsorption to alumina. Optical (absorbance and fluorescence) characterization of the dissolved organic matter samples demonstrates that (1) peak C (excitation 300-350 nm; emission 400-460 nm) fluorescence emission wavelength; (2) the ratio of peak T (excitation 220-235 nm; emission 330-370 nm) to peak C fluorescence intensity; and (3) the peak C fluorescence intensity: absorbance at 340 nm ratio have strong correlations with many of the functional assays. Strongest correlations are with benzo[a]pyrene binding, alumina adsorption, hydrophilicity and buffering capacity, and in many cases linear regression equations with a correlation coefficient >0.8 are obtained. These optical properties are independent of freshwater dissolved organic carbon concentration (for concentrations <10 mg L(-1)) and therefore hold the potential for laboratory, field and on-line monitoring and prediction of organic matter functional properties.
分析了来自三条河流和一个湖泊的25个溶解有机物样品的荧光激发-发射矩阵特性。所有采样点均进行了双份采样，25个样品包括从湖泊采样点采集的10个样品，以及从其中一条河流采集的9个样品，以涵盖因季节和河流流量导致的溶解有机物组成变化。将荧光特性与溶解有机物的功能特性进行比较；功能测定提供了关于光化学褪色、缓冲能力、铜结合、苯并[a]芘结合、亲水性和对氧化铝吸附的定量信息。溶解有机物样品的光学（吸光度和荧光）表征表明：（1）峰C（激发波长300 - 350 nm；发射波长400 - 460 nm）的荧光发射波长；（2）峰T（激发波长220 - 235 nm；发射波长330 - 370 nm）与峰C荧光强度的比值；以及（3）峰C荧光强度与340 nm处吸光度的比值与许多功能测定具有很强的相关性。最强的相关性存在于与苯并[a]芘结合、氧化铝吸附、亲水性和缓冲能力之间，并且在许多情况下获得了相关系数>0.8的线性回归方程。这些光学特性与淡水溶解有机碳浓度无关（对于浓度<10 mg L(-1)），因此具有在实验室、现场和在线监测及预测有机物功能特性的潜力。
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