Wang Jiang-tao, Guan Ha-si-gao-wa, Zhao Wei-hong, Wang Xiu-lin
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 May;29(5):1345-8.
Sea water samples were collected in the East China Sea in March and April, 2005, and three-dimensional fluorescence of dissolved organic matter was measured by fluorescence excitation-emission matrix spectroscopy. The position, number and intensity of fluorescence peak in the spectra and the relations of the peaks were analyzed to determine the type, distribution and origin of the fluorescence dissolved organic matter. Seven types of fluorescence peaks were detected from the samples. There are protein-like fluorescence peaks B with Exmax/Emmax = 275/300 nm, D with Exmax/Emmax = 225/295-305 nm, T with Exmax/Emmax = 280/345 nm, and S with Exmax/Emmax = 225-240/320-350 nm, two humic-like peaks A with Exmax/Emmax = 250-255/410-455 nm and C 335-345/410-440 nm, and marine humic peak M with Exmax/Emmax = 305 nm/400-420 nm. Peaks B, S and A appeared in all surveyed area. Peaks T and D appeared in the north of the surveyed area. Peaks M and C only appeared in a few stations. In the surface layer, the source of the fluorescence dissolved organic matter might be the fresh water outflow of the Yangtze River, while the fluorescence dissolved organic matter in the middle layer had double sources from the Yangtze River and the phytoplankton. The good correlationships of different fluorescence peaks showed the same source or some relationship between the protein-like and the humic-like fluorescence dissolved organic matter.
2005年3月和4月在中国东海采集海水样本，采用荧光激发-发射矩阵光谱法测量溶解有机物的三维荧光。分析光谱中荧光峰的位置、数量和强度以及峰之间的关系，以确定荧光溶解有机物的类型、分布和来源。从样本中检测到七种类型的荧光峰。有类蛋白质荧光峰B，Exmax/Emmax = 275/300 nm；D，Exmax/Emmax = 225/295 - 305 nm；T，Exmax/Emmax = 280/345 nm；S，Exmax/Emmax = 225 - 240/320 - 350 nm；两个类腐殖质峰A，Exmax/Emmax = 250 - 255/410 - 455 nm和C，335 - 345/410 - 440 nm；以及海洋腐殖质峰M，Exmax/Emmax = 305 nm/400 - 420 nm。峰B、S和A出现在所有调查区域。峰T和D出现在调查区域北部。峰M和C仅在少数站点出现。在表层，荧光溶解有机物的来源可能是长江的淡水流出，而中层的荧光溶解有机物有来自长江和浮游植物的双重来源。不同荧光峰之间的良好相关性表明类蛋白质和类腐殖质荧光溶解有机物有相同来源或某种关系。
