Minchin Stephen D, Busby Stephen J W
School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Methods. 2009 Jan;47(1):6-12. doi: 10.1016/j.ymeth.2008.10.012. Epub 2008 Oct 24.
In bacteria, the expression of transcription units is controlled by regulatory regions, that contain one or more promoters and binding sites for regulatory proteins that activate or repress expression in response to different signals. In this chapter, we explain the diverse approaches that can be used to understand the mechanisms by which the different factors intervene, and how the effects are integrated. Bioinformatics, genetics and biochemistry must be combined to understand the organisation of regulatory regions and the mechanisms by which transcription initiation is controlled.
在细菌中，转录单元的表达受调控区域控制，这些调控区域包含一个或多个启动子以及调控蛋白的结合位点，调控蛋白可根据不同信号激活或抑制表达。在本章中，我们将解释可用于理解不同因素干预机制以及这些效应如何整合的多种方法。必须将生物信息学、遗传学和生物化学结合起来，以了解调控区域的组织以及转录起始的控制机制。
