Datta Sibnarayan, Banerjee Arup, Chandra Partha Kumar, Biswas Avik, Panigrahi Rajesh, Mahapatra Pradip Kumar, Panda Chinmoy Kumar, Chakrabarti Shekhar, Bhattacharya Sujit Kumar, Chakravarty Runu
ICMR Virus Unit, Kolkata, ID and BG Hospital Campus, GB-4, 1st Floor (East Wing), 57, Dr. Suresh Chandra Banerjee Road, Pin-700010 (Beliaghata), Kolkata, West Bengal, India.
Virology. 2008 Dec 20;382(2):190-8. doi: 10.1016/j.virol.2008.09.007. Epub 2008 Oct 25.
HBx genetic variability was explored in the Eastern Indian population with low HCC incidence. DNase I sensitive HBV DNA was detected in 53% samples, which differed significantly between clinical groups (P<0.001). HBV genotypes A (Aa/A1), C (Cs/C1) and D (D1, D2, D3, D5) were detected in 37.5%, 18.7% and 43.7% samples respectively. Population specific signature HBx residues A(36), V(88), S(101) in Aa/A1 and residues P(41), Q(110) in D5 were detected. Mutations T(127), M(130) and I(131) were detected in 66.7%, 91% and 75% of genotype A, C and D5 samples respectively. Very low occurrence of HCC associated mutations (V(5)M/L, P(38)S, and H(94)Y) and absence of C-terminal deletions were observed. Our study shows that HBV genotype associated clinically important HBx variations may evolve and act distinctly in different geo-ethnic populations. Further studies on HBx functions from the perspective of genetic variability are essential for the better understanding of the clinical significance of HBV.
在印度东部肝癌发病率较低的人群中探索了HBx基因变异性。在53%的样本中检测到对DNase I敏感的HBV DNA，其在临床组之间存在显著差异（P<0.001）。分别在37.5%、18.7%和43.7%的样本中检测到HBV基因型A（Aa/A1）、C（Cs/C1）和D（D1、D2、D3、D5）。检测到Aa/A1中群体特异性标志性HBx残基A(36)、V(88)、S(101)以及D5中的残基P(41)、Q(110)。分别在66.7%、91%和75%的基因型A、C和D5样本中检测到突变T(127)、M(130)和I(131)。观察到肝癌相关突变（V(5)M/L、P(38)S和H(94)Y）的发生率极低且不存在C末端缺失。我们的研究表明，HBV基因型相关的具有临床重要性的HBx变异可能在不同的地理种族人群中演变并表现出明显差异。从基因变异性角度对HBx功能进行进一步研究对于更好地理解HBV的临床意义至关重要。
