Shah Z H, Harris S, Smith J L, Hodges E
Department of Molecular Pathology, Southampton General Hospital, Southampton, UK.
J Clin Pathol. 2009 Feb;62(2):177-81. doi: 10.1136/jcp.2007.054239. Epub 2008 Oct 24.
Angioimmunoblastic T cell lymphoma (AILT) is an aggressive T cell lymphoma with an incidence of approximately 1-2% of all non-Hodgkin lymphoma. The detection of clonal T cell receptor (TCR) gene rearrangements helps in the diagnosis of T cell malignancies such as AILT, where morphological and immunohistological investigations are not always sufficient to reach a definitive diagnosis. TCR beta (TCRB) and TCR gamma (TCRG) gene rearrangements were analysed from 17 WHO-defined cases of AILT by PCR for the presence of TCR clonality. TCRB gene rearrangements were sequenced to identify molecular signature(s) common among this patient group. Monoclonal and oligoclonal TCRB and TCRG gene rearrangements were detected in all cases. BV17S1 was slightly over-represented compared to the use of other Vbeta gene segments; however, no preferential usage of J gene segment(s) was detected. The results of this study emphasise that TCR clonality and oligoclonality is a diagnostic feature of AILT and that BV17S1 is over-represented with no other common molecular findings.
血管免疫母细胞性T细胞淋巴瘤（AILT）是一种侵袭性T细胞淋巴瘤，在所有非霍奇金淋巴瘤中的发病率约为1%-2%。克隆性T细胞受体（TCR）基因重排的检测有助于诊断AILT等T细胞恶性肿瘤，在这些疾病中，形态学和免疫组织学检查并不总是足以做出明确诊断。通过聚合酶链反应（PCR）分析了17例世界卫生组织（WHO）定义的AILT病例的TCRβ（TCRB）和TCRγ（TCRG）基因重排，以检测TCR的克隆性。对TCRB基因重排进行测序，以确定该患者群体中常见的分子特征。在所有病例中均检测到单克隆和寡克隆TCRB和TCRG基因重排。与其他Vβ基因片段的使用相比，BV17S1略有过度表达；然而，未检测到J基因片段的优先使用情况。本研究结果强调，TCR克隆性和寡克隆性是AILT的诊断特征，且BV17S1过度表达，无其他常见分子发现。
