Kim Susie, Moon Doo-Bum, Lee Chung-Hwan, Nam Soo-Wan, Kim Pil
Department of Biotechnology, Catholic University of Korea, Bucheon, Gyunggi, South Korea.
Curr Microbiol. 2009 Feb;58(2):159-63. doi: 10.1007/s00284-008-9294-6. Epub 2008 Oct 25.
To better understand how the reducing power of either NADH or NADPH affects cell growth, Escherichia coli strains expressing either NADH-dependent or NADPH-dependent azoreductase (EC 1.6.5.2), which mediates the reduction of an azo dye, were cultured in glucose minimal medium in the presence of 200 muM methyl red. Growth rates in NADH-perturbed, NADPH-perturbed, and control cells were 0.05, 0.12, and 0.13 h(-1), respectively. In addition, glucose consumption in NADH-perturbed cells was 10.8 g glucose/g cell, while that of control and NADPH-perturbed cells was very similar (3.6 vs 3.8 g glucose/g cell) during the perturbation phase. Therefore, NADH perturbation had a larger effect than NADPH on cellular growth.
为了更好地理解NADH或NADPH的还原能力如何影响细胞生长，将表达介导偶氮染料还原的NADH依赖性或NADPH依赖性偶氮还原酶（EC 1.6.5.2）的大肠杆菌菌株在含有200μM甲基红的葡萄糖基本培养基中培养。NADH干扰细胞、NADPH干扰细胞和对照细胞的生长速率分别为0.05、0.12和0.13 h⁻¹。此外，在干扰阶段，NADH干扰细胞的葡萄糖消耗量为10.8 g葡萄糖/g细胞，而对照细胞和NADPH干扰细胞的葡萄糖消耗量非常相似（分别为3.6 g葡萄糖/g细胞和3.8 g葡萄糖/g细胞）。因此，NADH干扰对细胞生长的影响比NADPH更大。
