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包含v-src癌基因的重组鼠逆转录病毒基因组的产生：一种诱导脑部血管肉瘤的病毒的分离。

Generation of recombinant murine retroviral genomes containing the v-src oncogene: isolation of a virus inducing hemangiosarcomas in the brain.

作者信息

Hevezi P, Goff S P

机构信息

Department of Microbiology, Columbia University, New York, New York 10032.

出版信息

J Virol. 1991 Oct;65(10):5333-41. doi: 10.1128/JVI.65.10.5333-5341.1991.

DOI:10.1128/JVI.65.10.5333-5341.1991
PMID:1895387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249013/
Abstract

A series of recombinant retroviral genomes was generated by cotransformation of NIH 3T3 cells with a mixture of cloned DNAs: a proviral copy of the wild-type Moloney murine leukemia virus, and Moloney-based vectors containing defective copies of the chicken v-src and the murine v-abl oncogenes. Morphologically transformed foci, appearing at low frequencies in these cultures, released high titers of transforming viruses. Analysis of one group of these viruses showed that the genomes were recombinants containing portions of the viral gag gene juxtaposed to the v-src oncogene. Biologically active cloned DNAs of two of these viruses were obtained and mapped in detail. One of these viruses did not cause disease after inoculation into newborn mice, but the other induced rapidly fatal hemangiosarcomas located exclusively in the brain.

摘要

通过将克隆的DNA混合物（野生型莫洛尼鼠白血病病毒的前病毒拷贝以及含有鸡v-src和鼠v-abl癌基因缺陷拷贝的基于莫洛尼病毒的载体）共转染NIH 3T3细胞，产生了一系列重组逆转录病毒基因组。在这些培养物中以低频率出现的形态转化灶释放出高滴度的转化病毒。对其中一组这些病毒的分析表明，基因组是重组体，包含与v-src癌基因并列的病毒gag基因部分。获得了其中两种病毒具有生物活性的克隆DNA并进行了详细定位。其中一种病毒接种新生小鼠后不引起疾病，但另一种则诱导仅位于大脑的快速致命性血管肉瘤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2f7/249013/a03e9ebe821e/jvirol00053-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2f7/249013/54bbb4cdd3b6/jvirol00053-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2f7/249013/11bde5377283/jvirol00053-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2f7/249013/a03e9ebe821e/jvirol00053-0223-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2f7/249013/54bbb4cdd3b6/jvirol00053-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2f7/249013/11bde5377283/jvirol00053-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d2f7/249013/a03e9ebe821e/jvirol00053-0223-a.jpg

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