[分子方法在细菌性眼内炎诊断中的应用]
[Application of molecular method in identification of bacterial endophthalmitis].
作者信息
Wu Wei, Lu Xin-xin, Huang Yan-fei
机构信息
Department of Clinical Laboratory, Beijing Tongren Hospital Affiliated to Capital University of Medical Sciences, Beijing 100730, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2008 May 27;88(20):1429-32.
OBJECTIVE
To search for a non-culture method to improve the efficiency of etiological diagnosis of infective endophthalmitis and other infectious diseases.
METHODS
Fifty clinical specimens of infective endophthalmitis were collected. DNA of bacterium was extracted directly from the aqueous fluid or vitreous fluid and then amplified with universal primers. The PCR products were sequenced subsequently.
RESULTS
Twenty-two (44%) bacterial smears and 13 (26%) cultured specimens were positive. PCR positivity was seen in 28 of the 50 cases (56%) and 22 specimens (44%) were sequenced successfully. The results of 16s rRNA gene sequence analysis did not completely conform to the results obtained from culture-dependent approach.
CONCLUSION
Molecular identification method is a rapid and efficient approach for the diagnosis of bacterial infective endophthalmitis.
目的
寻找一种非培养方法以提高感染性眼内炎及其他感染性疾病的病因诊断效率。
方法
收集50份感染性眼内炎的临床标本。直接从房水或玻璃体中提取细菌DNA，然后用通用引物进行扩增。随后对PCR产物进行测序。
结果
22份（44%）细菌涂片和13份（26%）培养标本呈阳性。50例中有28例（56%）PCR呈阳性，22份标本（44%）成功测序。16s rRNA基因序列分析结果与基于培养方法获得的结果不完全一致。
结论
分子鉴定方法是诊断细菌性感染性眼内炎的一种快速有效的方法。
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