通过聚合酶链反应和基因测序诊断的木糖氧化无色杆菌性眼内炎
Achromobacter xylosoxidans endophthalmitis diagnosed by polymerase chain reaction and gene sequencing.
作者信息
Uy Harvey S, Matias Ronald, de la Cruz Frederick, Natividad Filipinas
机构信息
Department of Ophthalmology, University of the Philippines, Philippine General Hospital, Manila, and Asian Eye Institute, Makati, Philippines.
出版信息
Ocul Immunol Inflamm. 2005 Dec;13(6):463-7. doi: 10.1080/09273940590951043.
PURPOSE
To report a patient with Achromobacter xylosoxidansendophthalmitis that was diagnosed using polymerase chain reaction and gene sequencing.
METHODS
Case report. A patient with culture-negative endophthalmitis underwent an anterior chamber tap. Polymerase chain reaction was performed on the aqueous sample using the 16S ribosomal DNA primer set to detect and amplify bacterial DNA. The amplified DNA was sequenced and compared to archived sequences in a gene library using the BLAST search program.
RESULTS
A 214-base pair gene sequence was amplified and matched with the gene sequence for A. xylosoxidans. Antimicrobial treatment was instituted with resolution of hypopyon, anterior chamber cells, and vitreous cells one month after treatment.
CONCLUSIONS
Polymerase chain reaction of 16S rDNA combined with gene sequencing may be an alternative method of diagnosing culture-negative bacterial endophthalmitis.
目的
报告一例通过聚合酶链反应和基因测序诊断的木糖氧化无色杆菌性眼内炎患者。
方法
病例报告。一名培养结果为阴性的眼内炎患者接受了前房穿刺。使用16S核糖体DNA引物对水样进行聚合酶链反应，以检测和扩增细菌DNA。对扩增的DNA进行测序，并使用BLAST搜索程序与基因库中的存档序列进行比较。
结果
扩增出一条214个碱基对的基因序列，与木糖氧化无色杆菌的基因序列匹配。治疗一个月后，给予抗菌治疗，前房积脓、前房细胞和玻璃体细胞消退。
结论
16S rDNA聚合酶链反应结合基因测序可能是诊断培养结果为阴性的细菌性眼内炎的一种替代方法。
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