Zhang Yan-qiong, Wang Yue-xiang, Xu Ge-zhi, Song Hou-yan, Wang Wen-ji
Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai 200031, China.
Zhonghua Yan Ke Za Zhi. 2008 May;44(5):448-54.
To study the development and opsin expression of zebrafish photoreceptors after CRX gene knock-down.
It was a experimental study. CRX-MOs was microinjected into the zygote of zebrafish. Seventy-two and 96 hours post fertilization, we prepared the eye histological slides, electron microscope samples and whole mount in-situ hybridizations to study the development and opsin expression. of photoreceptors.
There were no obvious histological changes in optical microscope observation after CRX-MOs microinjection. Outer segments were developed in control and blank groups, but no obvious outer segment was found in CRX group by electron microscope observation. Opsin Rho, SWS1 and SWS2 were expressed in control and blank groups and the expression of these genes was obviously decreased in CRX group.
CRX gene can influence the development and opsins expression of photoreceptors outer segments in zebrafish.
研究CRX基因敲低后斑马鱼光感受器的发育及视蛋白表达情况。
本研究为实验性研究。将CRX-MOs显微注射到斑马鱼的受精卵中。在受精后72小时和96小时，制备眼部组织学切片、电子显微镜样本和整体原位杂交，以研究光感受器的发育及视蛋白表达。
显微注射CRX-MOs后，光学显微镜观察未发现明显的组织学变化。对照组和空白组的外段发育正常，但电子显微镜观察发现CRX组未出现明显的外段。视蛋白Rho、SWS1和SWS2在对照组和空白组中表达，而这些基因在CRX组中的表达明显降低。
CRX基因可影响斑马鱼光感受器外段的发育及视蛋白表达。
