Pisarev M A, de Pisarev D L
Acta Endocrinol (Copenh). 1977 Feb;84(2):297-302. doi: 10.1530/acta.0.0840297.
Thyrotrophin (TSH) regulates the biosynthesis of thyroid protein and RNA. This action is mediated by adenylate cyclase and cycl AMP. In the present study the action of cyclic GMP and cyclic CMP was investigated in beef slices. Both cyclic AMP and cyclic GMP significantly increased the incorporation of [3H]uridine into RNA. These effects were blocked by actinomycin D, suggesting that their action is located at a preor at a transcriptional step. The PCA soluble fraction radioactivity followed in parallel with tha variations observed in the RNA fraction, supporting the view that cyclic nucleotides may regulate RNA by modulating the nucleotide precursors pool. Cyclic CMP in concentrations between 0.35 to 1.5 mM progressively decreased the RNA labelling, and the values of the PCA soluble radioactivity again followed those of RNA. Furthermore, cyclic CMP also blocked the in vitro stimulatory action of cyclic AMP on the incorporation of [3H]leucine into protein, and of [3H]uridine into RNA. The present results provide the first information on the action of cyclic AMP on RNA synthesis and suggest that negative signals may also play a part in the regulation of thyroid function.
促甲状腺激素（TSH）调节甲状腺蛋白和RNA的生物合成。这一作用由腺苷酸环化酶和环磷酸腺苷（cAMP）介导。在本研究中，对环磷酸鸟苷（cGMP）和环磷酸胞苷（cCMP）在牛肉切片中的作用进行了研究。cAMP和cGMP均显著增加了[3H]尿苷掺入RNA的量。这些作用被放线菌素D阻断，表明它们的作用位于转录前或转录步骤。三氯乙酸（PCA）可溶性部分的放射性与RNA部分观察到的变化平行，支持环核苷酸可能通过调节核苷酸前体池来调节RNA的观点。浓度在0.35至1.5 mM之间的cCMP逐渐降低RNA标记，PCA可溶性放射性值再次与RNA的放射性值一致。此外，cCMP还阻断了cAMP对[3H]亮氨酸掺入蛋白质以及[3H]尿苷掺入RNA的体外刺激作用。本研究结果首次提供了关于cAMP对RNA合成作用的信息，并表明负信号也可能在甲状腺功能调节中发挥作用。
