Sastry K V, Agrawal V P, Garg V K
Acta Histochem. 1976;57(2):183-90. doi: 10.1016/S0065-1281(76)80046-3.
The distribution and histochemical localization of G6Pase and 5-nucleotidase in the different parts of the alimentary canal of two teleost fishes, Heteropneustes fossilis and Barbus sophore have been studied. The major sites of activity of the two enzymes are intestinal mucosa and liver. G6Pase is localized in the cytoplasm of the absorptive cells of the intestinal mucosa while 5-nucleotidase is present in the cytoplasm as well as nucleus and cell membrane. In the intestine, the anterior portion shows more stronger activity than in the middle and posterior portions. Mild activity is also noticed in the mucosa and gastric glands of the stomach. G6Pase activity is stronger than 5-nucleotidase activity in all the portions. Weak 5-nucleotidase activity is found in the submucosal connective tissue and the nuclei of the intestine. The goblet cells, muscularis abd serosa are negative in all the portions. G6Pase activity is stronger in the herbivorous fish Barbus sophore than in the omnivorous form Heteropneustes fossilis. In the liver, stronger activity of two enzymes is localized in the hepatocytes surrounding the sinusoids. Connective tissue and endothelial lining are negative.
对两种硬骨鱼——印度胡鲶和苏氏野鲮消化道不同部位葡萄糖-6-磷酸酶（G6Pase）和5-核苷酸酶的分布及组织化学定位进行了研究。这两种酶的主要活性部位是肠黏膜和肝脏。G6Pase定位于肠黏膜吸收细胞的细胞质中，而5-核苷酸酶存在于细胞质、细胞核和细胞膜中。在肠道中，前部的活性比中部和后部更强。在胃的黏膜和胃腺中也观察到轻微活性。在所有部位，G6Pase的活性都强于5-核苷酸酶的活性。在黏膜下层结缔组织和肠细胞核中发现微弱的5-核苷酸酶活性。在所有部位，杯状细胞、肌层和浆膜均呈阴性。草食性鱼类苏氏野鲮的G6Pase活性比杂食性鱼类印度胡鲶更强。在肝脏中，两种酶的较强活性定位于血窦周围的肝细胞中。结缔组织和内皮衬里呈阴性。
