Ochi J
Department of Urology, Okayama University Medical School.
Nihon Hinyokika Gakkai Zasshi. 1991 Jul;82(7):1045-52. doi: 10.5980/jpnjurol1989.82.1045.
A cis-diamminedichloroplatinum (CDDP)-resistant cell line, CL-7/CDDP, has been established by chronic exposure of a parent human bladder cancer cell line, T24 (CL-7), to CDDP at progressively increased concentrations for 20 months. CL-7/CDDP was 2.7-fold more resistant to CDDP than CL-7 as determined by colony formation assay. Biological and biochemical characteristics were examined in CL-7/CDDP to compare with CL-7. There were no differences in doubling time and plating efficiency between the two cell lines, but a small one in the mode of chromosome numbers. Intra-cellular CDDP amounts after CDDP exposure showed no difference between the two cell lines, but CL-7/CDDP showed a higher glutathione (GSH) and metallothionein (MT) level. It is suggested that the mechanism of acquired resistance to CDDP is attributed to intra-cellular detoxication, but not to the efflux. CL-7/CDDP may be useful for elucidation of the mechanism of CDDP resistance in human bladder cancer.
通过将亲本人类膀胱癌细胞系T24（CL-7）在20个月内逐步暴露于浓度逐渐增加的顺铂（CDDP）中，建立了一种顺铂耐药细胞系CL-7/CDDP。通过集落形成试验测定，CL-7/CDDP对CDDP的耐药性是CL-7的2.7倍。检测了CL-7/CDDP的生物学和生化特性以与CL-7进行比较。两种细胞系在倍增时间和平板接种效率上没有差异，但在染色体数目模式上有微小差异。CDDP暴露后细胞内CDDP含量在两种细胞系之间没有差异，但CL-7/CDDP显示出更高的谷胱甘肽（GSH）和金属硫蛋白（MT）水平。提示获得性CDDP耐药机制归因于细胞内解毒作用，而非外排作用。CL-7/CDDP可能有助于阐明人类膀胱癌中CDDP耐药的机制。
