一种类似毛细胞白血病的新细胞系（ESKOL）的特征：癌基因调控和晚期B细胞分化的模型

Characterization of a new cell line (ESKOL) resembling hairy-cell leukemia: a model for oncogene regulation and late B-cell differentiation.

作者信息

Harvey W, Srour E F, Turner R, Carey R, Maze R, Starrett B, Kanagala R, Pereira D, Merchant P, Taylor M

机构信息

Department of Biology, Earlham College, Richmond, IN 47374.

出版信息

Leuk Res. 1991;15(8):733-44. doi: 10.1016/0145-2126(91)90077-7.

DOI:10.1016/0145-2126(91)90077-7

PMID:1895754

Abstract

A B-lymphoblastoid cell line ESKOL, composed of differentiated cells resembling hairy-cell leukemia (HCL) has been established from the peripheral blood (PB) of a HCL patient. Morphologically, ESKOL cells share several features with HCL B cells. Flow cytometric analysis revealed that ESKOL cells express HC2, CD21, PCA-1, CD24, FMC7, and CD25. Analysis by Northern-blot hybridization indicated that cultured cells expressed the oncogenes c-myc, H-ras and c-fos. RNA from 3T3 cells transfected with ESKOL DNA hybridized with H-ras and c-fos DNA probes. The ESKOL cells cultured in the presence of increasing concentrations, of alpha interferon demonstrated a decrease in the rate of cellular growth and an increase in the expression of CD21, CD25, FMC7 and PCA-1. Scanning electron microscopy revealed that cells incubated in the presence of alpha interferon underwent membranous changes with a loss of villosity. These observations suggest that IFN tends to drive HC out of their developmental arrest towards maturation.

摘要

一种名为ESKOL的B淋巴母细胞系，由类似于毛细胞白血病（HCL）的分化细胞组成，已从一名HCL患者的外周血（PB）中建立。从形态学上看，ESKOL细胞与HCL B细胞有几个共同特征。流式细胞术分析显示，ESKOL细胞表达HC2、CD21、PCA-1、CD24、FMC7和CD25。Northern印迹杂交分析表明，培养的细胞表达癌基因c-myc、H-ras和c-fos。用ESKOL DNA转染的3T3细胞的RNA与H-ras和c-fos DNA探针杂交。在α干扰素浓度不断增加的情况下培养的ESKOL细胞显示细胞生长速率下降，CD21、CD25、FMC7和PCA-1的表达增加。扫描电子显微镜显示，在α干扰素存在下孵育的细胞发生膜性变化，绒毛消失。这些观察结果表明，干扰素倾向于促使毛细胞从发育停滞状态走向成熟。