血红素与转化生长因子-β对HEL红白血病细胞中血红蛋白积累的协同作用。
The synergistic effect of hemin and transforming growth factor-beta on hemoglobin accumulation in HEL erythroleukemia cells.
作者信息
Hooper W C, Pruckler J, Jackson D, Evatt B L
机构信息
Division of Immunologic, Oncologic and Hematologic Diseases, Centers for Disease Control, Atlanta, GA 30333.
出版信息
Leuk Res. 1991;15(8):753-8. doi: 10.1016/0145-2126(91)90079-9.
Transforming growth factor-beta (TGF beta) and hemin can both independently induce hemoglobin accumulation in the human HEL erythroleukemia cell line. The combination of these two agents resulted in a synergistic effect in the production of hemoglobin. On day 1, following exposure to both hemin and TGF beta, approximately 35% of the cells had accumulated hemoglobin, as evidenced, by benzidine staining. Whereas, when treated alone with either agent, the percentage of benzidine-positive cells was less than 10%. By day 5, approximately 70-80% on the cells treated with the combination were benzidine-positive. Cell surface analysis showed that the combination of TGF beta and hemin increased the expression of CD34, CD64, glycophorin A, and GPIIb-IIIa(CDW41). Cell proliferation was decreased by the combination.
转化生长因子-β（TGF-β）和血红素均可独立诱导人HEL红白血病细胞系中血红蛋白的积累。这两种因子的组合在血红蛋白的产生中产生了协同效应。在第1天，在同时暴露于血红素和TGF-β后，约35%的细胞积累了血红蛋白，联苯胺染色证明了这一点。然而，当单独用任何一种因子处理时，联苯胺阳性细胞的百分比均小于10%。到第5天，用该组合处理的细胞中约70-80%为联苯胺阳性。细胞表面分析表明，TGF-β和血红素的组合增加了CD34、CD64、血型糖蛋白A和糖蛋白IIb-IIIa（CDW41）的表达。该组合降低了细胞增殖。
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