Qin Shu-Lan, Li Tao-Sheng, Kubo Masayuki, Ohshima Mako, Furutani Akira, Hamano Kimikazu
Department of Surgery and Clinical Science, Yamaguchi University, Graduate School of Medicine, Ube, Japan.
Circ J. 2008 Dec;72(12):2075-80. doi: 10.1253/circj.cj-08-0392. Epub 2008 Oct 29.
Implantation of bone marrow cells (BMCs) is a treatment of ischemic disease. It is well known that many inflammatory cytokines are released in ischemic tissue, especially in the acute phase, so in the present study it was investigated if the transient increase of cytokines in the acute ischemic tissue influences cell-based therapeutic angiogenesis.
Ischemic limb models were created in C57BL/6 mice as 24 h (acute) or 2 weeks (chronic) after ischemia. BMCs were cultured with total tissue protein, which was extracted from the acute and chronic ischemic muscles. The survival, adhesion, and migration of BMCs were significantly better after culture with 1 mg/ml total tissue protein extracted from the acute ischemic limbs than from the chronic ischemic limbs (p<0.001). For the in-vivo study, 8 x 10(6) BMCs, collected from green fluorescent protein (GFP) transgenic mice, were implanted into the acute or chronic ischemic limbs of the mice. The survival of implanted cells and blood flow were significantly better when BMCs were implanted into the acute ischemic limbs than into the chronic ischemic limbs (p<0.001).
A transient increase of cytokines in the acute ischemic tissue is beneficial for cell-based therapeutic angiogenesis.
骨髓细胞（BMCs）植入是一种治疗缺血性疾病的方法。众所周知，在缺血组织中会释放许多炎性细胞因子，尤其是在急性期，因此在本研究中，探讨了急性缺血组织中细胞因子的短暂增加是否会影响基于细胞的治疗性血管生成。
在C57BL/6小鼠中建立缺血肢体模型，分别在缺血后24小时（急性）或2周（慢性）。将BMCs与从急性和慢性缺血肌肉中提取的总组织蛋白一起培养。与从慢性缺血肢体中提取的1mg/ml总组织蛋白培养相比，与从急性缺血肢体中提取的1mg/ml总组织蛋白培养后，BMCs的存活、黏附和迁移明显更好（p<0.001）。对于体内研究，从绿色荧光蛋白（GFP）转基因小鼠收集的8×10⁶个BMCs被植入小鼠的急性或慢性缺血肢体。当BMCs植入急性缺血肢体时，植入细胞的存活和血流量明显优于植入慢性缺血肢体时（p<0.001）。
急性缺血组织中细胞因子的短暂增加有利于基于细胞的治疗性血管生成。
