卵泡刺激素自身抗体与特发性生精功能障碍有关。

Jian Wang Bing Yao, Liang Wei, Cui Ying-Xia, Ge Yi-Feng

Institute of Clinical Laboratory Medicine, Nanjing Jinling Hospital, Clinical School of Medical College, Nanjing University, Nanjing 210002, China.

Asian J Androl. 2008 Nov;10(6):915-21. doi: 10.1111/j.1745-7262.2008.00441.x.

AIM

To detect the anti-follicle-stimulating hormone (FSH) antibody in idiopathic infertile patients and fertile subjects in order to determine the role of this antibody in patients with spermatogenic dysfunction.

METHODS

The anti-FSH antibody in serum was detected by an enzyme-linked immunosorbent assay (ELISA). The functional and structural integrity of the sperm membrane was evaluated with hypo-osmotic swelling (HOS) test and the ultrastructure of the spermatozoa was investigated by transmission electron microscopy (TEM).

RESULTS

The extent of positive FSH antibody in the patients with oligozoospermia and/or asthenozoospermia was significantly higher than that in the fertile subjects and infertile patients with normal sperm concentration and motility, but it was significantly lower than that in the patients with azoospermia. The extent of anti-FSH antibody in the patients with azoospermia was significantly greater than that in patients with oligospermia and/or asthenospermia, infertile people with normal sperm density and motility and fertile people. The hypo-osmotic swelling test showed that the percentage of HOS-positive spermatozoa (swollen) was 45.1 mu 3.5% in the FSH antibody-positive group and 59.1% micro 6.2% in the FSH antibody-negative control group. The percentage of functional membrane damage to spermatozoa was significantly higher in the anti-FSH antibody-positive group than in the control group. TEM showed that the outer acrosomal membrane was located far from the nucleus, and detachment of the acrosome was found in the FSH autoantibody-positive group.

CONCLUSION

These data suggest that the presence of anti-FSH antibody is strongly correlated with the sperm quantity and quality in idiopathic male infertility. Anti-FSH antibody may be an important factor causing spermatogenic dysfunction and infertility.

目的

检测特发性不育患者及正常生育者体内抗促卵泡生成素（FSH）抗体，以确定该抗体在生精功能障碍患者中的作用。

方法

采用酶联免疫吸附测定（ELISA）法检测血清中的抗FSH抗体。用低渗肿胀试验（HOS）评估精子膜的功能和结构完整性，并用透射电子显微镜（TEM）研究精子的超微结构。

结果

少精子症和/或弱精子症患者中FSH抗体阳性程度显著高于正常生育者及精子浓度和活力正常的不育患者，但显著低于无精子症患者。无精子症患者中抗FSH抗体程度显著高于少精子症和/或弱精子症患者、精子密度和活力正常的不育者及正常生育者。低渗肿胀试验显示，FSH抗体阳性组中HOS阳性精子（肿胀）的百分比为45.1%±3.5%，FSH抗体阴性对照组为59.1%±6.2%。抗FSH抗体阳性组中精子功能膜损伤的百分比显著高于对照组。TEM显示，顶体膜外层远离细胞核，在FSH自身抗体阳性组中发现顶体分离。