Yao Bing, Zhang Leilei, Cui Yingxia, Wang Yongmei, Huang Yufeng
Laboratory of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, PLA4, Nanjing, Jiangsu 210002, China.
Zhonghua Nan Ke Xue. 2004 Jul;10(7):491-2, 495.
To detect the anti-FSH antibody using ELISA, and further probe into the role of anti-FSH in infertile patients.
The anti-FSH antibody was detected using ELISA in the serum of patients with spermatogenesis dysfunction, of infertile patients with normal sperm density and motility, and of normal fertile males.
The positive rate of anti-FSH antibody in the patients with oligospermia and/or asthenospermia [22.4% (22/98)] was significantly higher than that in the normal fertile [4% (2/50)] (P < 0.05) and that in the infertile patients with normal sperm density and motility [6.7% (2/30)] (P < 0.05). The positive rate of anti-FSH antibody in the patients with oligospermia and/or asthenospermia was lower than that in the patients with azoospermia [54.5% (12/22)] (P < 0.05). There was no significant difference in the positive rate between the normal control and the sterile males with normal sperm density and motility.
The anti-FSH antibody may be an important factor to cause spermatogenesis dysfunction by combining FSH to form immune compound and depress the activation of FSH.
采用酶联免疫吸附测定法（ELISA）检测抗促卵泡生成素（FSH）抗体，并进一步探讨抗FSH在不孕患者中的作用。
运用ELISA法检测精子发生功能障碍患者、精子密度及活力正常的不孕患者以及正常生育男性血清中的抗FSH抗体。
少精子症和/或弱精子症患者中抗FSH抗体阳性率[22.4%（22/98）]显著高于正常生育男性[4%（2/50）]（P<0.05），也显著高于精子密度及活力正常的不孕患者[6.7%（2/30）]（P<0.05）。少精子症和/或弱精子症患者中抗FSH抗体阳性率低于无精子症患者[54.5%（12/22）]（P<0.05）。正常对照组与精子密度及活力正常的不育男性之间阳性率无显著差异。
抗FSH抗体可能是通过与FSH结合形成免疫复合物并抑制FSH的激活，从而导致精子发生功能障碍的一个重要因素。
