Stanelle Rayman D, Marcus R Kenneth
Department of Chemistry, Biosystems Research Complex, Clemson University, Clemson, SC, 29634-1905, USA.
Anal Bioanal Chem. 2009 Jan;393(1):273-81. doi: 10.1007/s00216-008-2457-2. Epub 2008 Oct 29.
Nylon-6 capillary-channeled polymer (C-CP) fibers are used as the stationary phase for the hydrophobic interaction chromatography (HIC) separation of a synthetic protein mixture composed of ribonuclease A, lysozyme, and holotransferrin. Nylon is a useful polymer phase for HIC as it has an alkyl backbone, while the amide functionality is hydrophilic (in fact ionic) in nature. The combination of a nonporous polymer surface of the fiber phases and high column permeability yields very efficient mass transfer characteristics, as exhibited by narrowing of peak widths with increases in mobile phase linear velocity. Retention factors and resolution were evaluated at flow rates ranging from 0.5 to 9 mL/min (linear velocities of ca. 2 to 15 mm/s) and at gradient slopes between 3.3 and 20 %B/min. Optimum resolution was achieved by employing fast flow rates (9 mL/min) and slow gradients (3 %B/min), also resulting in the highest peak capacities.
尼龙-6毛细管通道聚合物（C-CP）纤维用作固定相，用于对由核糖核酸酶A、溶菌酶和全转铁蛋白组成的合成蛋白质混合物进行疏水相互作用色谱（HIC）分离。尼龙是一种适用于HIC的聚合物相，因为它具有烷基主链，而酰胺官能团本质上是亲水的（实际上是离子性的）。纤维相的无孔聚合物表面与高柱渗透性相结合，产生了非常高效的传质特性，这表现为随着流动相线性速度的增加峰宽变窄。在流速范围为0.5至9 mL/min（线性速度约为2至15 mm/s）以及梯度斜率在3.3至20%B/min之间时，对保留因子和分离度进行了评估。通过采用快速流速（9 mL/min）和缓慢梯度（3%B/min）实现了最佳分离度，同时也得到了最高的峰容量。
