Bugelski Peter J, Kim Carin
Toxicology and Investigational Pharmacology, Centocor Research and Development, Radnor, Pennsylvania 19355, USA.
J Immunotoxicol. 2007 Apr;4(2):159-64. doi: 10.1080/15476910701337126.
The primary T-cell-dependent antibody response (TDAR) to antigens is widely used as a functional test in immunotoxicology. TDAR assays can be performed using different antigens, e.g., sheep red blood cells (SRBC) and keyhole limpet hemocyanin (KLH) are presently utilized by different laboratories. The purpose of this study was to apply meta-analysis to compare data from several laboratories using these antigens, in two strains of rats, by multiple routes of immunization and with different primary TDAR assay formats. Although limitations imposed by the scale of the data set and the need to convert some data to an ordinal scale to allow comparisons restrict the scope and strength of the conclusions that can be drawn, the results of this study have shown that the two antigens in different assay formats give comparable results. Similarly, both antigens and assays formats showed the same pattern of response to strong immunotoxicants. These results indicate that standardizing the choice of antigen in TDAR assays may not be critical in the evaluation of potential immunotoxicity.
针对抗原的主要T细胞依赖性抗体反应（TDAR）在免疫毒理学中被广泛用作功能测试。TDAR检测可使用不同抗原进行，例如，目前不同实验室使用绵羊红细胞（SRBC）和钥孔血蓝蛋白（KLH）。本研究的目的是应用荟萃分析，比较在两种品系大鼠中，通过多种免疫途径并采用不同的原发性TDAR检测形式，使用这些抗原的多个实验室的数据。尽管数据集规模带来的限制以及为便于比较将一些数据转换为有序尺度的需求限制了可得出结论的范围和力度，但本研究结果表明，不同检测形式中的两种抗原给出了可比结果。同样，抗原和检测形式对强免疫毒剂均呈现相同的反应模式。这些结果表明，在TDAR检测中标准化抗原选择对于潜在免疫毒性评估可能并非关键。
