Sun Hui, Chan King Yee, Fung Ying Sing
Department of Chemistry, Hong Kong University, Hong Kong SAR, China.
Electrophoresis. 2008 Oct;29(19):3971-9. doi: 10.1002/elps.200800082.
A new continuous-flow gradient-elution micellar electrokinetic capillary chromatography method is developed for the determination of airborne carbonyls after derivatization with 2,4-dinitrophenylhydrazine. A total of 16 carbonyls can be determined with detection limits ranging from 0.94 to 8.50 mg/L, working range from 4.72 to 346 mg/L, and repeatabilities (relative standard deviation, n=5) from 1.23 to 4.6% or 3.93 to 7.6% for migration time and peak area, respectively. Coupling with denuder-filter sampling, a preliminary survey has been conducted to determine gaseous and particulate carbonyls from air sampled at a roadside station. The method is shown to have sufficient sensitivity for 1-h sampling of ambient carbonyls with detection limits ranging from 0.045 to 1.2 microg/m3 and working range from 0.11 to 43.3 microg/m3 at a flow rate of 10 Lpm. The method requires minimal modification of commercially available capillary electrophoresis equipment and can differentiate gaseous and particulate carbonyls to provide essential information and objective data for adopting effective measures to combat the discharge of carbonyl compounds to the atmosphere.
建立了一种新的连续流动梯度洗脱胶束电动毛细管色谱法，用于测定经2,4-二硝基苯肼衍生化后的空气中羰基化合物。共可测定16种羰基化合物，检测限为0.94至8.50mg/L，工作范围为4.72至346mg/L，迁移时间和峰面积的重复性（相对标准偏差，n = 5）分别为1.23%至4.6%或3.93%至7.6%。结合扩散管-过滤器采样，对路边站点采集的空气中气态和颗粒态羰基化合物进行了初步调查。结果表明，该方法对环境羰基化合物1小时采样具有足够的灵敏度，在流速为10Lpm时，检测限为0.045至1.2μg/m³，工作范围为0.11至43.3μg/m³。该方法只需对市售毛细管电泳设备进行最小程度的改装，并且能够区分气态和颗粒态羰基化合物，为采取有效措施应对羰基化合物向大气中的排放提供重要信息和客观数据。
