Sorokin A V, Noniashvili E M, Kidgotko O V, Sasina L K, Aleĭnikova T D, Shavlovskiĭ M M, Gorodetskiĭ S I, Dyban A P
Tsitologiia. 2008;50(9):773-9.
One of the crucial problems of developmental biology is the study of mechanisms of regulation of gene expression in early embryogenesis. Here we studied dynamics of mosaic appearance of a marker fluorescent protein in in vitro developing mice embryo derived from zygotes after microinjections to male pronuclei of cloned DNA fragment carrying EGFP under control of different promoters. Main attention was paid to initial stages of development, when structural rearrangements and reprogramming of both parental genomes, activation of zygotic genes, and control of development by embryo genome take place.
发育生物学的关键问题之一是研究早期胚胎发生过程中基因表达调控机制。在此，我们研究了在体外发育的小鼠胚胎中，将携带在不同启动子控制下的EGFP的克隆DNA片段显微注射到雄原核后，标记荧光蛋白镶嵌出现的动态过程。主要关注发育的初始阶段，此时双亲基因组会发生结构重排和重编程、合子基因激活以及胚胎基因组对发育的控制。
