Bodi Z, Gysler-Junker A, Kohli J
Department of Genetics, Lajos Kossuth University, Debrecen, Hungary.
Mol Gen Genet. 1991 Sep;229(1):77-80. doi: 10.1007/BF00264215.
The fidelity of mitotic chromosome transmission in Schizosaccharomyces pombe was estimated quantitatively by using cycloheximide resistance as a means to select cells that had undergone chromosome loss or nondisjunction. We aimed to investigate the connection between recombination and mitotic chromosome stability. A number of mutants defective in mitotic recombination such as cdc17-L16, rec59-72, and rec50-25 were tested and in these an approximately ten fold elevation of mitotic haploidization rate was found compared with controls. Our data suggest that recombination is important in controlling the maintenance of chromosomes during mitosis.
通过使用环己酰亚胺抗性作为选择经历了染色体丢失或不分离的细胞的手段，对粟酒裂殖酵母有丝分裂染色体传递的保真度进行了定量估计。我们旨在研究重组与有丝分裂染色体稳定性之间的联系。测试了许多有丝分裂重组缺陷的突变体，如cdc17-L16、rec59-72和rec50-25，发现这些突变体的有丝分裂单倍体化率比对照高约十倍。我们的数据表明，重组在有丝分裂期间控制染色体的维持中很重要。
