Phull M, Nigam P C
Department of Chemistry, Indian Institute of Technology, Kanpur 208016, India.
Talanta. 1983 Jun;30(6):401-4. doi: 10.1016/0039-9140(83)80094-0.
Some sulphur-containing ligands have been shown to inhibit the Hg(II)-catalysed substitution of p-nitrosodiphenylamine (p-NDA) for cyanide in hexacyanoferrate(II), by binding the mercury(II). This effect is used for determination of microamounts of cysteine, thioglycollic acid and thiosulphate. The reactions are followed spectrophotometrically at 640 nm (lambda(max) of Fe(CN)(5).p-NDA). The determination range depends on the amount of mercury(II) added and the stability of the Hg(II)-ligand complex. Under specified conditions, the detection limits are: thioglycollic acid 1 x 10(-7)M, cysteine 1 x 10(-6)M and thiosulphate 4 x 10(-7)M.
一些含硫配体已被证明可通过与汞（II）结合来抑制汞（II）催化的对亚硝基二苯胺（p-NDA）在亚铁氰化物（II）中取代氰化物的反应。这种效应被用于测定微量的半胱氨酸、巯基乙酸和硫代硫酸盐。反应在640nm处用分光光度法进行跟踪（Fe(CN)(5).p-NDA的λ(max)）。测定范围取决于所添加汞（II）的量以及汞（II）-配体络合物的稳定性。在特定条件下，检测限为：巯基乙酸1×10(-7)M、半胱氨酸1×10(-6)M和硫代硫酸盐4×10(-7)M。
